Identification and Characterization of a Homologue to the Arabidopsis INDEHISCENT Gene in Common Bean
Identification and Characterization of a Homologue to the Arabidopsis INDEHISCENT Gene in Common Bean
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DOI：
10.1093/jhered/ess102
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发表时间：
2013-03-01
影响因子：
3.1
通讯作者：
Gepts, Paul
中科院分区：
文献类型：
--
作者：
Gioia, Tania;Logozzo, Giuseppina;Gepts, Paul
减少豆荚破碎是菜豆驯化综合症的关键组成部分，并使这种驯化依赖于农民进行种子传播。试图阐明这一过程的遗传控制导致了一个主效基因(ST)的鉴定，该基因与影响豆荚破碎的角果缝合纤维的存在/不存在有关。虽然ST基因已被放入菜豆遗传图谱，但该基因的序列和具体功能尚不清楚。本研究的目的是在拟南芥中找到一个候选基因，INDEHISCENT基因(IND)是角果破碎所需的初级工厂。利用两个重组自交系群体(BAT93 JALO EEP558和MIDAS G12873)，在普通菜豆中成功扩增出与IND同源的序列，并将其置于菜豆图谱上。尽管PvIND位于ST位点附近，但PvIND和ST之间没有完全的共分离，以及与裂开/不裂表型相关的PvIND基因座缺乏多态性，这表明PvIND可能不直接参与豆荚破碎，也可能不是ST基因座的基础基因。然而，PvIND可能与ST基因座上一个尚未确定的调控元件密切相关。或者，需要开发一种考虑到数量性状变异的更精确的表型方法，以更准确地定位ST基因座。
Reduction in pod shattering represents a key component of the domestication syndrome in common bean (Phaseolus vulgaris) and makes this domesticate dependent upon the farmer for seed dispersal. Attempts to elucidate the genetic control of this process have led to the identification of a major gene (St) linked to the presence/absence of pod suture fibers affecting pod shattering. Although St has been placed on the common bean genetic map, the sequence and the specific functions of this gene remain unknown. The purpose of this study was to identify a candidate gene for St. In Arabidopsis thaliana, INDEHISCENT gene (IND) is the primary factory required for silique shattering. A sequence homologous to IND was successfully amplified in P. vulgaris and placed on the common bean map using two recombinant inbred populations (BAT93 Jalo EEP558; Midas G12873). Although PvIND maps near the St locus, the lack of complete cosegregation between PvIND and St and the lack of polymorphisms at the PvIND locus correlated with the dehiscent/indehiscent phenotype suggests that PvIND may not be directly involved in pod shattering and may not be the gene underlying the St locus. However, PvIND may be closely linked to an as yet unidentified regulatory element at the St locus. Alternatively, a more precise phenotyping method taking into account quantitative trait variation needs to be developed to more accurately map the St locus.