Genome-wide identification, phylogeny and function analysis of GRAS gene family in Dendrobium catenatum (Orchidaceae).
Genome-wide identification, phylogeny and function analysis of GRAS gene family in Dendrobium catenatum (Orchidaceae).
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DOI：
10.1016/j.gene.2019.04.038
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发表时间：
2019-07
期刊：
影响因子：
3.5
通讯作者：
Xu Zeng;H. Ling;Xiao-mei Chen;Shun‐xing Guo
中科院分区：
文献类型：
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作者：
Xu Zeng;H. Ling;Xiao-mei Chen;Shun‐xing Guo
研究背景近年来，植物生长发育的分子机制得到了广泛的研究。GRAS基因是植物特有的转录因子家族，在这一过程中起着关键作用。GRAS转录因子与腋芽分生组织的形成、辐射状根的形成、植物激素（赤霉素）的信号转导、光信号以及非生物或生物胁迫等有关。结果根据与拟南芥和水稻GRAS家族成员的系统发育关系，共鉴定出47个链霉菌GRAS基因，并将其编码的蛋白分为11个亚类。这些GRAS基因大多包含一个外显子，并且系统发育树中密切相关的成员具有相似的基序组成。我们的研究结果还表明，GRAS基因在链霉菌中广泛分布，并在不同的组织中表达。此外，从不同亚群中成功克隆了35个GRAS基因，并利用大肠杆菌系统诱导表达了7个DoGRAS融合蛋白。此外，8个基因在不同的组织中表达上调，暴露于热和盐stress.ConclusionOur研究结果提供了有价值的信息和候选基因，为未来的功能分析，以提高抗链霉菌生长。
BackgroundIn recent years, the molecular mechanism of plant growth and development has been reported in detail. GRAS genes, a plant-specific family of transcription factor, play critical roles in the process. GRAS transcription factors are associated with axillary shoot meristem formation, radial root patterning, phytohormones (gibberellins) signal transduction, light signaling, and abiotic or biotic stress.ObjectiveHere, we firstly investigated GRAS gene family in Dendrobiumcatenatum, an important medicinal and flowering orchid in China.MethodsThe GRAS gene family inD.catenatumwas cloned based on RNA-Seq data. Selected GRAS genes were introduced intoEscherichia colito express proteins.ResultsBased on phylogenetic relationship with the Arabidopsis andOryzaGRAS family members, 47 GRAS genes fromD.catenatumare identified and their deduced proteins are classified into 11 subgroups. Most of these GRAS genes contain one exon and closely related members in the phylogenetic tree have similar motif composition. Our result also reveals that GRAS genes inD.catenatumare widely distributed and expressed in different tissue. In addition, 35 GRAS genes are successfully cloned from different subgroups and 7 DoGRAS fusion proteins are induced usingE.colisystem. Moreover, 8 genes were up-regulated in different tissue following exposure to heat and salt stresses.ConclusionOur findings provide valuable information and candidate genes for future functional analysis for improving the resistance ofD.catenatumgrowth.