Genome-wide Insertional mutagenesis of Arabidopsis thaliana
Genome-wide Insertional mutagenesis of Arabidopsis thaliana
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DOI：
10.1126/science.1086391
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发表时间：
2003-08-01
期刊：
影响因子：
56.9
通讯作者：
Ecker, JR
中科院分区：
文献类型：
--
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
已经建立了超过225,000个独立的农杆菌转移的DNA（T-DNA）插入事件，该插入事件已创建了拟南芥的基因组，代表了基因空间的饱和度接近饱和。确定了超过88,000个T-DNA插入的精确位置，这导致了21,700多个类似于29,454个预测的拟南芥基因的突变。整个整合事件分布的全基因组分析揭示了在染色体和基因水平上都存在较大的整合位点偏差。在响应于植物激素乙烯的基因中鉴定了插入突变。
Over 225,000 independent Agrobacterium transferred DNA (T-DNA) insertion events in the genome of the reference plant Arabidopsis thaliana have been created that represent near saturation of the gene space. The precise locations were determined for more than 88,000 T-DNA insertions, which resulted in the identification of mutations in more than 21,700 of the similar to29,454 predicted Arabidopsis genes. Genome-wide analysis of the distribution of integration events revealed the existence of a large integration site bias at both the chromosome and gene levels. Insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene.