Erythrocyte osmotic fragility test for screening of alpha-thalassemia-1 and beta-thalassemia trait in pregnancy
Erythrocyte osmotic fragility test for screening of alpha-thalassemia-1 and beta-thalassemia trait in pregnancy
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DOI：
10.1016/j.ijgo.2004.04.037
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发表时间：
2004-09-01
影响因子：
3.8
通讯作者：
Tongsong, T
中科院分区：
文献类型：
--
作者：
Sirichotiyakul, S;Tantipalakorn, C;Tongsong, T
目的：评价渗透脆性试验（OFT）作为重型地中海贫血（α-地中海贫血-1 β-地中海贫血）筛查试验的敏感性和特异性。研究方法：一个描述性分析和诊断测试的非贫血孕妇参加产前保健诊所，Maharaj Nakom清迈，在2002年4月和7月。收集446例无明显医学并发症如缺铁性贫血的单胎妊娠的血样。在同一天使用0.45%甘油盐水溶液进行OFT，将小于60%的临界值用于异常检测。以HbA 2定量检测和PCR（SEA型）作为金标准，分别确诊β-地中海贫血和α-地中海贫血1型。结果：OFT的敏感性、特异性、阳性预测值和阴性预测值为主要指标。如果OFT临界值小于60%被认为是阳性，则该测试的灵敏度、特异性、阳性和阴性预测值分别为97.6%、72.9%、33.6%和99.5%。结论：OFT在检测α-地中海贫血-1性状或β-地中海贫血性状方面具有高灵敏度，并且由于其简单性和非常低的成本，因此可以被认为是广泛人群中的筛查试验。(C)2004年国际妇产科联合会。由Elsevier爱尔兰有限公司出版。保留所有权利。
Objectives: To evaluate the sensitivity and specificity of osmotic fragility test (OFT) as a screening test in predicting the severe thalassemia trait (alpha-thalassemia-1 beta-thalassemia). Methods: A descriptive analysis and diagnostic test of non-anemic pregnant women attending antenatal care clinic, Maharaj Nakom Chiang Mai, during April, and July 2002 was made. Blood samples were collected from 446 singleton pregnancies with no obvious medical complication such as iron deficiency anemia. OFT was performed in the same day, using 0.45% glycerin saline solution and the cut-off value of less than 60% was used for an abnormal test. Quantitative HbA2 test and PCR (SEA type) were done as a gold standard to confirm the diagnosis of beta-thalassemia trait and alpha-thalassemia-1 trait, respectively. Results: The main outcome measures were sensitivity, specificity, positive and negative predictive value of OFT. If the OFT cut-off value of less than 60% was considered positive, the test had a sensitivity, specificity, positive and negative predictive value of 97.6%, 72.9%, 33.6%, and 99.5%, respectively. Conclusion: OFT has high sensitivity in detection of alpha-thalassemia-1 trait or beta-thalassemia trait and due to its simplicity with very low cost it may, therefore, be considered as a screening test in a wide population. (C) 2004 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.