CENTRAL INTERLEUKIN-1-BETA ENHANCES SPLENIC SYMPATHETIC-NERVE ACTIVITY IN RATS
CENTRAL INTERLEUKIN-1-BETA ENHANCES SPLENIC SYMPATHETIC-NERVE ACTIVITY IN RATS
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DOI：
10.1016/0361-9230(94)90139-2
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发表时间：
1994-01-01
影响因子：
3.8
通讯作者：
HORI, T
中科院分区：
文献类型：
--
作者：
ICHIJO, T;KATAFUCHI, T;HORI, T
免疫细胞因子如白细胞介素-1（IL-1）和干扰素-α（IFN-α）的中枢给药导致外周细胞免疫的抑制，这至少部分取决于交感神经活性。在乌拉坦和铜氯醛糖麻醉的大鼠中，第三脑室（I3 V）内输注重组人IL-1 β（rhIL-1 β）（1-5 ng/大鼠）引起脾交感神经电活动的剂量依赖性增加。rhIL-1 β（1 ng/rat）的作用可通过预先使用IL-1受体拮抗剂（1 μ g/rat，在rhIL-1 β前10 min I3 V）、水杨酸钠（1 μ g/rat）或铜-黑素细胞刺激激素（cu-MSH）（400 ng/rat）完全阻断。此外，促肾上腺皮质激素释放因子（CRF）的拮抗剂，α-螺旋CRF（9-41）（2 μ g/大鼠），完全消除rhIL-1 β诱导的脾神经活性增加，尽管CRF的I3 V输注这些结果表明，脑中的IL-1 β通过其受体介导的和前列腺素介导的激活脾交感神经活动。对a-MSH敏感的依赖性作用，具体取决于CRF系统。我们的研究结果，加上以前的结果，表明脾交感神经代表的沟通渠道之一，从大脑到免疫系统。
The central administration of immune cytokines such as interleukin-1 (IL-1) and interferon-alpha (IFN-alpha) results in the suppression of peripheral cellular immunity, which depends, at least partly, on the sympathetic nervous activity. An intrathird cerebroventricular (I3V) infusion of recombinant human IL-1 beta (rhIL-1 beta) (1-5 ng/rat) elicited a dose dependent increase in the electrical activity of the splenic sympathetic nerve in urethane and cu-chloralose anesthetized rats. The effect of rhIL-1 beta (1 ng/rat) was completely blocked by pretreatment with an IL-1 receptor antagonist (1 mu g/rat, I3V 10 min before rhIL-1 beta), sodium salicylate (1 mu g/rat), or cu-melanocyte stimulating hormone (cu-MSH) (400 ng/rat). Furthermore, an antagonist of corticotropin-releasing factor (CRF), alpha-helical CRF(9-41) (2 mu g/rat), completely abolished the rhIL-1 beta-induced increase in the splenic nerve activity, although an I3V infusion of CRF(1 mu g/rat) excited it. These results suggest that IL-1 beta in the brain activates splenic sympathetic activity by its receptor-mediated and prostaglandin-dependent action that is sensitive to a-MSH, depending on CRF system. Our findings, together with the previous results, suggest that the splenic sympathetic nerve represents one of the communication channels from the brain to the immune system.