Zebrafish neurobehavioral phenomics applied as the behavioral warning methods for fingerprinting endocrine disrupting effect by lead exposure at environmentally relevant level (vol 231, pg 315, 2019)
Zebrafish neurobehavioral phenomics applied as the behavioral warning methods for fingerprinting endocrine disrupting effect by lead exposure at environmentally relevant level (vol 231, pg 315, 2019)
复制标题
斑马鱼神经行为表型组学作为行为预警方法，用于指纹识别环境相关水平下铅暴露造成的内分泌干扰效应（第 231 卷，第 315 页，2019 年）
DOI：
10.1016/j.chemosphere.2020.127783
复制
发表时间：
2020
期刊：
影响因子：
8.8
通讯作者：
Jin Meng
中科院分区：
文献类型：
--
作者：
Li Xiang;Zhang Baoyue;Li Ning;Ji Xiuna;Liu Kechun;Jin Meng
环境铅（Pb）暴露对公众健康有很大危害。尽管环境相关的铅中毒是可以预防的，但潜在的铅污染物仍然是对人类健康的主要威胁。在此，我们报道了环境相关浓度水平（1μg/L、10μg/L和100μg/L）的Pb暴露扰乱了成年雄性斑马鱼的求偶行为，并进一步改变了参与睾丸类固醇生成的关键基因（igf3、amh、piwil1、lhcgr、fshr、cyp11c1、star、cyp19a1a、 cyp19a1b）和细胞凋亡（bax、cytoC、caspase 9、caspase 3、puma）。行为和转录特征都具有相似的双相剂量反应，低水平暴露后具有刺激作用，高水平暴露后具有抑制作用。该结果揭示了即使在环境相关水平内的环境水质标准（AWQC）浓度范围内，Pb也会产生内分泌干扰作用，并且运动指纹作为检测Pb污染引起的风险指标的可靠性。目前的研究首次采用ZebraTower系统作为生物预警系统（BEWS），发现Pb对雄性成年斑马鱼的求偶行为和内分泌调节产生双相效应。在方法上，我们首先提出了一种结合BEWS和斑马鱼表型组学等数据分析方法来监测和评估铅暴露风险的有效解决方案，这将为环境相关铅中毒的检测和预防做出贡献。
Environmental lead (Pb) exposure is a great hazard to the public health. Although environmentally relevant Pb poisoning is preventable, insidious Pb contaminants are still a major threat to human health. Herein, we reported that exposure to Pb at environmentally relevant concentration level (1 μg/L, 10 μg/L and 100 μg/L), disturbed the courtship behavior of adult male zebrafish and further altered the transcriptional patterns of key genes involved in testicular steroidogenesis (igf3,amh,piwil1,lhcgr, fshr, cyp11c1, star,cyp19a1a, cyp19a1b) and apoptosis (bax, cytoC,caspase 9,caspase 3, puma). Both the behavioral and the transcriptional profiles share a similar biphasic dose response, with stimulatory effects after low-level exposure and inhibitory effects after high-level exposure. This results revealed the endocrine disrupting effects of Pb even at an environmentally relevant level within the concentration range of ambient water quality criteria (AWQC) and the reliability of locomotion fingerprint as the indicator for detecting the risk induced by Pb pollution. Current research, for the first time, employed the ZebraTower system as the biological early warning system (BEWS) to find that Pb exerted biphasic effects on the courtship behavior and endocrine regulation of male adult zebrafish. Methodologically, we firstly propose an efficient solution to monitor and assess the risk of Pb exposure by combining the (BEWS) and data analyzing methods such as zebrafish phenomics, which would make a contributiontothe detection and prevention of environmentally relevant Pb poisoning.