Anatomical landmarks for registration of experimental image data to volumetric rodent brain atlasing templates
Anatomical landmarks for registration of experimental image data to volumetric rodent brain atlasing templates
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DOI：
10.1016/j.jneumeth.2014.11.005
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发表时间：
2015-01
影响因子：
3
通讯作者：
M. Sergejeva;Eszter A. Papp;Rembrandt Bakker;M. Gaudnek;Y. Okamura-Oho;J. Boline;J. Bjaalie;A. Hess
中科院分区：
文献类型：
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作者：
M. Sergejeva;Eszter A. Papp;Rembrandt Bakker;M. Gaudnek;Y. Okamura-Oho;J. Boline;J. Bjaalie;A. Hess
背景解剖参考的分配是整合迅速扩大的啮齿动物脑数据集合的关键步骤。基于地标的配准有助于不同类型数据的空间锚定，而不适合于对基于体素的图像信息进行操作的自动化方法。在这里，我们提出了一组标准化的解剖学标志，用于配准来自小鼠和大鼠大脑的全脑成像数据集，特别是用于在WaxholmSpace(WHS)中集成实验图像数据。结果C57BL/6J小鼠大脑的16个内部标志被可靠地识别：不同的个体，独立于他们的解剖经验；跨越不同的MRI对比度(T1、T2、T2*)和其他方式(Nissl组织学和块面解剖)；在不同的标本中；在不同的切片获取角度；在不同的图像分辨率下。利用这些标志点，我们给出了一个T1加权MRI与小鼠WHS模板之间的配准实例，并达到了相当高的准确率。鼠标WHS模板中确定的地标位置通过可伸缩大脑地图集共享，并附有定位每个地标的图形和文本指南。我们为Spraogue Dawley大鼠确定了WHS模板中的16个地标中的14个。与现有方法相比，该标志点集可以承受采集角度、成像方式等方面的实质性差异，并且不容易受到主观性的影响。结论这有助于将多模式3D脑数据注册到小鼠和大鼠脑的标准坐标空间，朝着创建共同的啮齿动物参考系统迈出了一步；将数据共享提高到了一个更高的质量水平。
Background Assignment of anatomical reference is a key step in integration of the rapidly expanding collection of rodent brain data. Landmark-based registration facilitates spatial anchoring of diverse types of data not suitable for automated methods operating on voxel-based image information. New tool Here we propose a standardized set of anatomical landmarks for registration of whole brain imaging datasets from the mouse and rat brain, and in particular for integration of experimental image data in Waxholm Space (WHS). Results Sixteen internal landmarks of the C57BL/6J mouse brain have been reliably identified: by different individuals, independent of their experience in anatomy; across different MRI contrasts (T 1, T 2, T 2*) and other modalities (Nissl histology and block-face anatomy); in different specimens; in different slice acquisition angles; and in different image resolutions. We present a registration example between T 1-weighted MRI and the mouse WHS template using these landmarks and reaching fairly high accuracy. Landmark positions identified in the mouse WHS template are shared through the Scalable Brain Atlas, accompanied by graphical and textual guidelines for locating each landmark. We identified 14 of the 16 landmarks in the WHS template for the Sprague Dawley rat. Comparison with existing methods This landmark set can withstand substantial differences in acquisition angle, imaging modality, and is less vulnerable to subjectivity. Conclusions This facilitates registration of multimodal 3D brain data to standard coordinate spaces for mouse and rat brain taking a step toward the creation of a common rodent reference system; raising data sharing to a qualitatively higher level.