Identification of genetic loci and candidate genes related to β-glucan content in barley grain by genome-wide association study in International Barley Core Selected Collection
Identification of genetic loci and candidate genes related to β-glucan content in barley grain by genome-wide association study in International Barley Core Selected Collection
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通过国际大麦核心精选集全基因组关联研究鉴定与大麦籽粒β-葡聚糖含量相关的遗传位点和候选基因
DOI：
10.1007/s11032-020-01199-5
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发表时间：
2021-01-13
影响因子：
3.1
通讯作者：
Zhang, Guoping
中科院分区：
文献类型：
--
作者：
Geng, La;Li, Mengdi;Zhang, Guoping
-葡聚糖是大麦育种计划中需要改进的重要性状，因为当大麦谷物用作饲料或麦芽生产的原料或作为人类食物消费时，它极大地影响最终产品的质量。虽然与β -葡聚糖合成相关的基因已经被确定，但大麦籽粒中β -葡聚糖积累的遗传调控仍然完全不清楚。以100份国际大麦核心精选选育材料（BCS）为研究对象，在2种环境下连续栽培2年，测定籽粒β -葡聚糖含量的基因型变异。一项全基因组关联研究（GWAS）鉴定出谷物β -葡聚糖含量与14个稳定的标记-性状关联（mta） （-Log10(P) bbbb4）。籽粒β -葡聚糖含量与14个稳定mta的有利等位基因数量呈极显著正相关。七个可能的候选基因编码一些葡萄糖代谢酶被发现与β -葡聚糖含量有关。其中一个推测基因HORVU6Hr1G088380可能是一个重要的控制β -葡聚糖含量的基因，其snp在所有测试材料中都紧密相连，并分为两个单倍型。高分辨率熔融（HRM）分析表明，该标记可用于大麦育种的标记辅助选择。该研究为大麦籽粒β -葡聚糖含量的相关基因和标记提供了有用的信息。
beta -glucan is an important trait to be improved in barley breeding programs, as it greatly affects the quality of the end products when barley grains are used as raw material of feed or malt production or consumed as food for human. Although the genes associated with beta -glucan synthesis have been identified, genetic regulation of beta -glucan accumulation in barley grains is still completely unclear. In this study, 100 accessions from International Barley Core Selected Collection (BCS) were planted in two environments for two consecutive years to determine the genotypic variation of grain beta -glucan content. A genome-wide association study (GWAS) identified 14 stable marker-trait associations (MTAs) (-Log10(P)>4) for grain beta -glucan content. Significantly positive correlation was found between grain beta -glucan content and the number of favorable alleles of 14 stable MTAs. Seven putative candidate genes encoding some enzymes in glucose metabolism were found to be associated with beta -glucan content. One of the putative genes, HORVU6Hr1G088380, could be an important gene controlling barely beta -glucan content, with the SNPs being closely linked in all tested accessions and divided into two haplotypes. High resolution melting (HRM) analysis of the first SNP suggested that the HRM-SNP marker is valid for marker-assisted selection in barley breeding. This study provides useful information for the genes and markers related to grain beta -glucan content in barley.