The plant host Brassica napus induces in the pathogen Verticillium longisporum the expression of functional catalase peroxidase which is required for the late phase of disease.
The plant host Brassica napus induces in the pathogen Verticillium longisporum the expression of functional catalase peroxidase which is required for the late phase of disease.
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DOI：
10.1094/mpmi-08-11-0217
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发表时间：
2012-03
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影响因子：
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通讯作者：
Seema Singh;S. Braus-Stromeyer;Christian Timpner;O. Valerius;A. von Tiedemann;P. Karlovsky;Christine Druebert;A. Polle;G. Braus
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文献类型：
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作者：
Seema Singh;S. Braus-Stromeyer;Christian Timpner;O. Valerius;A. von Tiedemann;P. Karlovsky;Christine Druebert;A. Polle;G. Braus
毁灭性的土传真菌病原体长孢轮枝菌是十字花科成员的特有宿主，其中包括作为经济上最重要的作物的油菜（Brassica napus）。该真菌通过根部感染，导致易受影响的寄主植物发育迟缓和早衰，并导致作物产量显着下降。我们在此表明，长孢葡萄球菌对甘蓝型油菜木质部汁液的存在作出反应，产生六种不同的上调蛋白质和八种下调蛋白质，通过二维凝胶电泳可视化。通过质谱鉴定 10 种蛋白质表明，所有上调的蛋白质都参与氧化应激反应。 V. longisporum 过氧化氢酶过氧化物酶 (VlCPEA) 是上调程度最高的蛋白质，由两个同基因 VlcpeA-1 和 VlcpeA-2 编码。两个基因有 98% 相同，证实了真菌的二倍体或“两倍体”状态。两个 VlcpeA 基因的敲除都会使蛋白质表达降低 80%，并导致对活性氧的敏感性。虽然腐生生长和植物感染的初始阶段在表型上不受影响，但突变体无法进行疾病的后期阶段。我们认为过氧化氢酶过氧化物酶在保护真菌免受宿主植物在疾病晚期产生的氧化应激方面发挥作用。
The devastating soilborne fungal pathogen Verticillium longisporum is host specific to members of the family Brassicaceae, including oilseed rape (Brassica napus) as the economically most important crop. The fungus infects through the roots and causes stunting and early senescence of susceptible host plants and a marked decrease in crop yield. We show here that V. longisporum reacts to the presence of B. napus xylem sap with the production of six distinct upregulated and eight downregulated proteins visualized by two-dimensional gel electrophoresis. Identification of 10 proteins by mass spectrometry revealed that all upregulated proteins are involved in oxidative stress response. The V. longisporum catalase peroxidase (VlCPEA) was the most upregulated protein and is encoded by two isogenes, VlcpeA-1 and VlcpeA-2. Both genes are 98% identical, corroborating the diploid or "amphihaploid" status of the fungus. Knock downs of both VlcpeA genes reduced protein expression by 80% and resulted in sensitivity against reactive oxygen species. Whereas saprophytic growth and the initial phase of the plant infection were phenotypically unaffected, the mutants were not able to perform the late phases of disease. We propose that the catalase peroxidase plays a role in protecting the fungus from the oxidative stress generated by the host plant at an advanced phase of the disease.