Altered expression and localization of desmoglein 3 in esophageal squamous cell carcinoma
Altered expression and localization of desmoglein 3 in esophageal squamous cell carcinoma
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桥粒芯糖蛋白 3 在食管鳞状细胞癌中表达和定位的改变
DOI：
10.1016/j.acthis.2014.01.010
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发表时间：
2014-01-01
影响因子：
2.5
通讯作者：
Li, En-Min
中科院分区：
文献类型：
--
作者：
Fang, Wang-Kai;Chen, Bo;Li, En-Min
桥粒蛋白3(Desmoglein 3，DSG3)是桥粒细胞-细胞黏附结构中的一种跨膜型钙粘附素，在维持正常的上皮组织结构中起着重要作用。关于DSG3在癌症中的表达作用的报道很少，而且相互矛盾。本研究采用免疫组织化学方法检测DSG3在食管鳞癌中的表达和亚细胞定位，并与临床特征进行相关性分析。结果表明，在正常鳞状细胞上皮细胞中，DSG3在棘层有较强的免疫反应，且仅定位于细胞膜。食管鳞癌中DSG3的胞浆定位异常，与细胞分化程度相关(P=0.018)。最显著的是，在74.1%的肿瘤中，DSG3的表达上调，并且与区域淋巴结转移有关(P=0.036)。此外，在无淋巴结转移的患者中，DSG3的胞浆定位与预后不良相关(P=0.044)。这些结果提示DSG3参与食管癌的发生发展，DSG3的过度表达可能是食道癌侵袭性特征的重要因素。(C)2014年爱思唯尔股份有限公司。版权所有。
Desmoglein 3 (DSG3), a transmembrane cadherin of the desmosomal cell-cell adhesion structure, plays vital roles in the maintenance of normal epithelial tissue architecture. Reports implicating a role for DSG3 expression in cancer are few and contradictory. In this study, immunohistochemical staining was employed to investigate DSG3 expression and subcellular localization in esophageal squamous cell carcinoma (ESCC), and to correlate changes with clinical characteristics. Results indicate that in normal squamous cell epithelia, strong DSG3 immunoreactivity was observed in the Stratum spinosum, and localization occurred only at the cell membrane. In ESCC, DSG3 immunoreactivity displayed an abnormal cytoplasmic localization that was correlated with cell differentiation (P = 0.018). Most strikingly, in 74.1% of the tumors, DSG3 expression was up-regulated and correlated with regional lymph node metastasis (P = 0.036). Moreover, in patients without lymph node metastasis, cytoplasmic localization of DSG3 correlated with poor prognosis (P = 0.044). These results suggest that DSG3 is involved in the development of ESCC and imply that DSG3 overexpression is likely to be an essential contributor to the aggressive features of esophageal cancer. (C) 2014 Elsevier GmbH. All rights reserved.