Bacteriophage Inactivation at the Air-Water-Solid Interface in Dynamic Batch Systems
Bacteriophage Inactivation at the Air-Water-Solid Interface in Dynamic Batch Systems
复制标题
动态批量系统中空气-水-固体界面处的噬菌体灭活
DOI：
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发表时间：
1999
影响因子：
4.4
通讯作者：
M. Yates
中科院分区：
文献类型：
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作者：
S. Thompson;M. Yates
摘要噬菌体已被广泛用作人类肠道病毒的替代物，用于病毒转运和命运的许多研究。在本研究中，在一系列动态批实验中研究了三种噬菌体MS 2、R17和φX174的命运。MS 2和R17在分批实验中都容易失活，其中每种噬菌体的溶液通过填充有不同比例的玻璃和Teflon珠的管进行纯化。MS 2和R17失活是暴露于动态空气-水-固体界面处的破坏力的结果。然而，在类似的研究中，φX174没有经历失活，这表明这种噬菌体不会在空气-水界面处积累，或者不会以相同的方式受到界面力的影响。其他批次实验表明，在聚丙烯管中混合期间，随着溶液离子强度的升高，MS 2和R17的失活程度逐渐增加（φX174不受影响）。通过向MS 2和R17的悬浮液中加入吐温80，防止了噬菌体失活。我们的数据表明，在简单的动态批量实验中，病毒灭活取决于（i）动态空气-水-固体界面（其中固体是疏水表面）的存在，（ii）溶液的离子强度，（iii）溶液中表面活性化合物的浓度，以及（iv）所用病毒的类型。
ABSTRACT Bacteriophages have been widely used as surrogates for human enteric viruses in many studies on virus transport and fate. In this investigation, the fates of three bacteriophages, MS2, R17, and φX174, were studied in a series of dynamic batch experiments. Both MS2 and R17 readily underwent inactivation in batch experiments where solutions of each phage were percolated through tubes packed with varying ratios of glass and Teflon beads. MS2 and R17 inactivation was the result of exposure to destructive forces at the dynamic air-water-solid interface. φX174, however, did not undergo inactivation in similar studies, suggesting that this phage does not accumulate at air-water interfaces or is not affected by interfacial forces in the same manner. Other batch experiments showed that MS2 and R17 were increasingly inactivated during mixing in polypropylene tubes as the ionic strength of the solution was raised (φX174 was not affected). By the addition of Tween 80 to suspensions of MS2 and R17, phage inactivation was prevented. Our data suggest that viral inactivation in simple dynamic batch experiments is dependent upon (i) the presence of a dynamic air-water-solid interface (where the solid is a hydrophobic surface), (ii) the ionic strength of the solution, (iii) the concentration of surface active compounds in the solution, and (iv) the type of virus used.