Radially expanding transglial calcium waves in the intact cerebellum
Radially expanding transglial calcium waves in the intact cerebellum
复制标题
DOI：
10.1073/pnas.0809269106
复制
发表时间：
2009-03-03
影响因子：
11.1
通讯作者：
Wang, Samuel S. -H.
中科院分区：
文献类型：
--
作者：
Hoogland, Tycho M.;Kuhn, Bernd;Wang, Samuel S. -H.
多细胞胶质钙波可能局部调节神经活动或脑能量学。在这里，我们报告了在正常、完整的大脑中，一个扩散驱动的星形细胞信号，它在一个有限的体积内跨越了许多星形细胞过程，而没有完全包围任何一个细胞。利用双光子显微镜观察鼠脑小脑皮层，用荧光指示染料或钙传感器蛋白G-CaMP2标记，我们发现自发钙波填充伯格曼胶质突起的近似椭球结构域。波以4-11 μ m/s的速度在三维空间传播，直径约为50 μ m，在扩张过程中减慢，并被P2受体拮抗剂可逆地阻断。与ATP作为钙释放波的扩散性触发器的假设一致，ATP的局部喷射触发P2受体介导的波，不易重复激活。跨神经胶质波是嘌呤能信号在局部特异性作用下调节局部神经回路活动或能量的一种手段。
Multicellular glial calcium waves may locally regulate neural activity or brain energetics. Here, we report a diffusion- driven astrocytic signal in the normal, intact brain that spans many astrocytic processes in a confined volume without fully encompassing any one cell. By using 2-photon microscopy in rodent cerebellar cortex labeled with fluorescent indicator dyes or the calcium-sensor protein G-CaMP2, we discovered spontaneous calcium waves that filled approximately ellipsoidal domains of Bergmann glia processes. Waves spread in 3 dimensions at a speed of 4-11 mu m/s to a diameter of approximate to 50 mu m, slowed during expansion, and were reversibly blocked by P2 receptor antagonists. Consistent with the hypothesis that ATP acts as a diffusible trigger of calcium release waves, local ejection of ATP triggered P2 receptor-mediated waves that were refractory to repeated activation. Transglial waves represent a means for purinergic signals to act with local specificity to modulate activity or energetics in local neural circuits.