Connective tissue activation. XXVII. The behavior of skin fibroblasts from patients with scleroderma.
Connective tissue activation. XXVII. The behavior of skin fibroblasts from patients with scleroderma.
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结缔组织激活。
DOI：
10.1002/art.1780261109
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发表时间：
1983
影响因子：
--
通讯作者：
Castor,CW
中科院分区：
文献类型：
--
作者：
Cabral,A;Castor,CW
比较了4种正常（NF）和4种硬皮病皮肤成纤维细胞（SF）菌株的1）基础14 C-葡萄糖胺和35 SO 4-标记的糖胺聚糖（GAG）合成，2）对自体激素介质的反应性，3）最大刺激后的性能。在基础条件下，SF合成和分泌的放射性透明质酸是NF的2-3倍（P< 0.001）;凝胶色谱法测得的分子量相似，表明其为高分子量产物。SF对正常淋巴和血小板因子的反应基本上与NF一样。在两组细胞之间未观察到硫酸化GAG合成的一致定性或定量差异。将NF和SF与假“核心蛋白”（如对硝基苯基-β-D-木糖苷）孵育表明，核心蛋白的合成是限速的;在β-木糖苷存在下，SF和NF在SO 4-GAG链合成中同样容易。SF似乎在体外保留了许多代的部分活化状态，至少在透明质酸合成方面。
Four normal (NF) and 4 scleroderma skin fibro‐blast (SF) strains were compared with respect to 1) basal14C‐glucosamine and35SO4‐labeled glycosaminoglycan (GAG) synthesis, 2) responsiveness to autacoid mediators, and 3) performance following maximal stimulation. Under basal conditions, SF synthesized and secreted 2–3 times more radioactive hyaluronic acid than the NF (P< 0.001); molecular volume by gel chromatography was similar and suggested a high molecular weight product. SF were essentially as responsive to normal lymphoid and platelet factors as were NF. No consistent qualitative or quantitative differences in sulfated GAG synthesis were noted between the 2 groups of cells. Incubation of NF and SF with a false “core protein” such as p‐nitrophenyl‐β‐D‐xyloside suggested that synthesis of the core protein was rate limiting; SF and NF were equally facile in SO4‐GAG chain synthesis in the presence of a β‐xyloside. SF appear to retain in vitro a partially activated state for many generations, at least with respect to hyaluronic acid synthesis.