The effective concentration and exposure time of mitomycin-C for the inhibition of lens epithelial cell proliferation in rabbit eyes.
The effective concentration and exposure time of mitomycin-C for the inhibition of lens epithelial cell proliferation in rabbit eyes.
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DOI：
10.3349/ymj.2000.41.2.185
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发表时间：
2000-04
影响因子：
2.4
通讯作者：
Y. S. You;S. Lim;H. S. Chung;Y. G. Lee;C. Kim;Y. Hong
中科院分区：
文献类型：
--
作者：
Y. S. You;S. Lim;H. S. Chung;Y. G. Lee;C. Kim;Y. Hong
白内障手术后残留晶状体上皮细胞的增生被认为是后囊膜混浊的主要原因。为了评估丝裂霉素c预防后囊膜混浊的效果，我们测定了丝裂霉素c抑制兔晶状体上皮细胞增殖的有效浓度和暴露时间。将第四代兔晶状体上皮细胞保存1天，然后分别暴露于丝裂霉素c中1、2、3和5分钟。有9种不同浓度的丝裂霉素c， 2倍连续稀释。通过逆转录-聚合酶链反应检测lambda-crystalline mRNA的持续转录和限制性内切片段的多态性，证实了晶状体上皮细胞表型特性的维持。用3h -胸腺嘧啶掺入DNA检测细胞增殖。第四代细胞维持了lambda-crystalline mRNA的表达，表明它们在表型上是真实的晶状体上皮细胞。丝裂霉素- c的有效浓度和暴露时间分别为0.1 mg/ml 1分钟和2分钟，0.025 mg/ml 2分钟。通过这些结果，我们推测丝裂霉素c在相对较短的孵育时间内可用于临床预防白内障术后后囊膜混浊。
The proliferation of residual lens epithelial cells following cataract surgery is assumed to be a major cause of posterior capsular opacification. To assess the efficacy of mitomycin-C in preventing posterior capsular opacification, we determined the effective concentration and exposure time of mitomycin-C in inhibiting rabbit lens epithelial cell proliferation. The fourth-passaged rabbit lens epithelial cells were maintained for one day and then exposed to mitomycin-C for 1, 2, 3, and 5 minutes, respectively. There were 9 different plating concentrations of mitomycin-C with two-fold serial dilution. The maintenance of the phenotypic properties of lens epithelial cells was confirmed by continuous transcription of lambda-crystalline mRNA determined by reverse transcription-polymerase chain reaction and the polymorphism of the restriction fragment. Cell proliferation was assayed with 3H-thymidine incorporation into DNA. The fourth-passaged cells maintained the expression of lambda-crystalline mRNA, suggesting that they are phenotypically authentic lens epithelial cells. The effective concentrations and exposure time of mitomycin-C were 0.1 mg/ml for 1 minute and 2 minutes, and 0.025 mg/ml for 2 minutes. By these results, we postulated that mitomycin-C at relatively short incubation times could be clinically used for prevention of posterior capsular opacification after cataract surgery.