Mitotype-specific sequences related to cytoplasmic male sterility in Oryza species
Mitotype-specific sequences related to cytoplasmic male sterility in Oryza species
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DOI：
10.1007/s11032-013-9993-y
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发表时间：
2014-04
影响因子：
3.1
通讯作者：
Hongwei Xie;Jie Wang;Mingjuan Qian;Nengwu Li;Ying-guo Zhu;Shaoqing Li
中科院分区：
文献类型：
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作者：
Hongwei Xie;Jie Wang;Mingjuan Qian;Nengwu Li;Ying-guo Zhu;Shaoqing Li
植物线粒体基因组含有大量的线粒体型特异性序列（MSS），这些序列建立了与其他线粒体型不同的线粒体基因组结构。在水稻中，9个线粒体基因组已被测序，这为我们提供了可能性，表征水稻的MSS和探讨其与水稻细胞质雄性不育（CMS）的关系。因此，我们分析了CW-CMS、LD-CMS、WA-CMS、N和日本晴系的线粒体基因组，发现了57个MSS，大小范围为102至5，745 bp，总长度为92.4 kb。MSS占水稻线粒体基因组的14.5%以上，是影响线粒体基因组大小变异的重要因素。在所检测的MSS中，34个MSS在水稻品系间表现出多态性，其中14个MSS被进一步证实为CMS所特有。这包括九个特定于孢子体CMS的MSS，三个特定于配子体CMS，两个由所有类型的CMS共享。有趣的是，除了CMS基因orf（H）79和orf 352与某些MSS片段部分或完全重叠外，在CMS特异的MSS中还发现了另外10个功能未知的开放阅读框架，暗示它们可能在植物CMS中发挥作用。这些新发现为杂交水稻不育系的选育提供了新的分子工具。
Plant mitochondrial genomes contain a large number of mitotype-specific sequences (MSS) which establish a mitochondrial genome structure distinct from other mitotypes. In rice, nine mitochondrial genomes have been sequenced, which provides us with the possibility of characterizing the MSS of rice and probing their relationship to cytoplasmic male sterility (CMS) in rice. We therefore analyzed the mitochondrial genomes of CW-CMS, LD-CMS, WA-CMS, N and Nipponbare lines, and found 57 MSS with sizes ranging from 102 to 5,745 bp, and with an aggregate length of 92.4 kb. The MSS account for more than 14.5 % of the rice mitochondrial genome and are a significant contributing factor in the variation of mitochondrial genome sizes. Of the MSS tested, 34 MSS exhibited polymorphism among rice lines, and 14 MSS were further confirmed as being specific to CMS. This includes nine MSS specific to sporophytic CMS, three specific to gametophytic CMS, and two shared by all types of CMS. Interestingly, except for CMS genesorf(H)79andorf352which are partly or fully overlapping with some MSS fragments, there are ten more open reading frames of unknown function that were detected in CMS-specific MSS, hinting at their possible roles in plant CMS. These novel findings provide us with potential new molecular tools to direct the breeding of CMS lines in hybrid rice breeding programs.