Interaction of the MexA and MexB components of the MexAB-OprM multidrug efflux system of Pseudomonas aeruginosa:: Identification of MexA extragenic suppressors of a T578I mutation in MexB
Interaction of the MexA and MexB components of the MexAB-OprM multidrug efflux system of Pseudomonas aeruginosa:: Identification of MexA extragenic suppressors of a T578I mutation in MexB
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DOI：
10.1128/aac.49.10.4375-4378.2005
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发表时间：
2005-10-01
影响因子：
4.9
通讯作者：
Poole, K
中科院分区：
文献类型：
--
作者：
Nehme, D;Poole, K
MexB中的T578I突变破坏了该蛋白对抗菌素耐药性的贡献，并对其与MexA的相互作用产生负面影响。突变导致MexA (R221H, L245F， E254K和V259I) c端结构域单氨基酸改变，抑制了表达MexB（T5781）的铜绿假单胞菌菌株的抗菌敏感性，恢复了MexA与MexB（T578I）的相互作用。这些数据证实了MexA c端区域在MexB结合中的重要性，以及MexB T587I周围区域在MexA相互作用中的可能意义。
A T578I mutation in MexB compromised the protein's contribution to antimicrobial resistance and negatively impacted its interaction with MexA. Mutations causing single amino acid changes in the C-terminal domain of MexA (R221H, L245F, E254K, and V259I) suppressed the antimicrobial susceptibility of a MexB(T5781)-expressing Pseudomonas aeruginosa strain and restored a MexA interaction with MexB(T578I). These data confirm the importance of the MexA C-terminal region in MexB binding and the likely significance of the region surrounding T587I of MexB in MexA interaction.