Long terminal repeat enhancement of v-mos transforming activity: identification of essential regions
Long terminal repeat enhancement of v-mos transforming activity: identification of essential regions
复制标题
v-mos转化活性的长末端重复增强：关键区域的识别
DOI：
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发表时间：
1983
影响因子：
5.4
通讯作者：
G. V. Vande Woude
中科院分区：
文献类型：
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作者：
T. Wood;M. McGeady;D. Blair;G. V. Vande Woude
通过在相对于v-mos的5'或3'位置引入莫洛尼肉瘤病毒长末端重复序列（LTR）来增强含有莫洛尼肉瘤病毒v-mos序列的重组DNA克隆的转化效率。我们分析了在这些重组DNA克隆转化的细胞中表达的聚腺苷酸化RNA，并检查了DNA整合拷贝的结构完整性。在每种情况下，我们证明了存在的v-mos含有RNA转录的聚腺苷酸化的RNA，并表明这些RNA转录与转染的DNA的结构是一致的。来自这些转化细胞的DNA的分析表明，转染DNA内的v-mos和LTR序列的相对位置在整合的DNA拷贝中是保守的。这些结果表明，单个LTR可以从5'或3'相对位置成功地增强v-mos的转化活性。来自仅含有导入v-mos 3'的LTR部分的重组克隆的转染分析的结果表明，负责增强转化的LTR的必需区域是含有73-碱基对串联重复序列的LTR的独特3'序列内的区域。
The transforming efficiency of recombinant DNA clones containing the Moloney sarcoma virus v-mos sequence was enhanced by introducing the Moloney sarcoma virus long terminal repeat (LTR) in either the 5' or 3' position relative to v-mos. We analyzed the polyadenylated RNA expressed in cells transformed by these recombinant DNA clones and examined the structural integrity of integrated copies of the DNA. In each case, we demonstrated the presence of v-mos containing RNA transcripts in the polyadenylated RNA and showed that these RNA transcripts are consistent with the structure of the transfected DNA. The analysis of DNA from these transformed cells showed that the relative positions of the v-mos and LTR sequences within the transfected DNA were conserved in the integrated DNA copies. These results demonstrate that a single LTR can successfully enhance the transforming activity of v-mos from either a 5' or a 3' relative position. The results from the transfection analysis of recombinant clones containing only portions of the LTR introduced 3' to v-mos demonstrate that the essential region of the LTR responsible for the enhancement of transformation is a region within the unique 3' sequences of the LTR containing the 73-base-pair tandem repeat sequence.