Dynamic imaging of the effect of mesenchymal stem cells on osteoclast precursor cell chemotaxis for bone defects in the mouse skull
Dynamic imaging of the effect of mesenchymal stem cells on osteoclast precursor cell chemotaxis for bone defects in the mouse skull
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DOI：
10.1016/j.jds.2018.08.001
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发表时间：
2018-12-01
影响因子：
3.5
通讯作者：
Tanimoto, Kotaro
中科院分区：
文献类型：
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作者：
Abe, Takaharu;Sumi, Keisuke;Tanimoto, Kotaro
背景/目的：间充质干细胞（MSC）移植此前已应用于再生医学领域。尽管已知骨再生是通过成骨细胞和破骨细胞之间的相互作用发生的，但间充质干细胞对破骨细胞的影响尚不清楚。因此，本研究的目的是探讨间充质干细胞对破骨细胞前体细胞（RAW264巨噬细胞）趋化性的影响。 材料与方法：在小鼠颅骨中制造骨缺损，将间充质干细胞和碳酸羟基磷灰石支架移植到骨缺损内。将RAW264细胞移植至小鼠尾静脉，通过体内成像系统观察其动态。结果：MSCs移植组在骨缺损区域的荧光强度在第3、5、7天明显高于MSCs非移植组。结论：在颅骨缺损处同时植入MSCs后，RAW264细胞对骨缺损区域的趋化性增强。 (C) 2018中华民国齿科医学会。 Elsevier B.V. 的出版服务。这是一篇遵循 CC BY-NC-ND 许可的开放获取文章。
Background/purpose: Mesenchymal stem cells (MSCs) transplantation has previously been used in the field of regenerative medicine. Although bone regeneration is known to occur through the interaction between osteoblasts and osteoclasts, the effect of MSCs on osteoclasts is unknown. Therefore, the purpose of this study was to investigate the effect of MSCs on the chemotaxis of osteoclast precursor cells (RAW264 macrophage cells).Materials and methods: Bone defects were created in mice skulls, and MSCs and a scaffold of carbonated hydroxyapatite were transplanted into the bone defects. RAW264 cells were then transplanted into the mouse tail vein, and their dynamics were observed by an in vivo imaging system.Results: The fluorescent intensity of the MSCs transplant group at the bone defect region was significantly higher on days 3, 5, and 7 compared with the MSCs non-transplant group.Conclusion: Increased RAW264 chemotaxis to the bone defect region occurred following the simultaneous implantation of MSCs in the skull defect. (C) 2018 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V. This is an open access article under the CC BY-NC-ND license.