DETERMINATIONS OF CITRIC-ACID BY DIFFERENTIAL PULSE POLAROGRAPHY WITH IMMOBILIZED ENZYMES
DETERMINATIONS OF CITRIC-ACID BY DIFFERENTIAL PULSE POLAROGRAPHY WITH IMMOBILIZED ENZYMES
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DOI：
10.1007/bf00332259
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发表时间：
1990-01-01
期刊：
影响因子：
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通讯作者：
YOSHIDA, H
中科院分区：
文献类型：
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作者：
HASEBE, K;HIKAMA, S;YOSHIDA, H
柠檬酸裂解酶和草酰乙酸脱羧酶可以快速、简单地固定在聚丙烯酰胺凝胶中，以提供选择性和灵敏的反应器。在最佳条件下柠檬酸向丙酮酸的转化率可达90%。生成的丙酮酸可以通过微分脉冲极谱法测定。该生物反应器可在几周内进行三十次测定。运动饮料和葡萄酒样品无需预处理即可成功检测；相对标准偏差为1.0倍。极谱溶液中10-5 mol l-1柠檬酸含量为1.17%，检出限为6.6倍。 10-7 mol l-1 (κ = 2)。
Citrate lyase and oxaloacetate decarboxylase can be rapidly and simply immobilized in polyacrylamide gel to provide a selective and sensitive reactor. Conversion ratio of citric acid to pyruvic acid comes up to 90% under the optimal conditions. Generated pyruvic acid can be determined by differential pulse polarography. The bioreactor can be used for thirty determinations over several weeks. Samples of sports drinks and wine were successfully tested without pretreatment; the relative standard deviation with 1.0 .times. 10-5 mol l-1 citric acid in polarographic solutions was 1.17% and the detection limit was 6.6 .times. 10-7 mol l-1 (.kappa. = 2).