Production of trout offspring from triploid salmon parents
Production of trout offspring from triploid salmon parents
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DOI：
10.1126/science.1145626
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发表时间：
2007-09-14
期刊：
影响因子：
56.9
通讯作者：
Yoshizaki, Goro
中科院分区：
文献类型：
--
作者：
Okutsu, Tomoyuki;Shikina, Shinya;Yoshizaki, Goro
许多鲑鱼已经面临灭绝的危险。对于卵子不能冷冻保存的硬骨鱼来说，开发卵子冷冻以外的技术来节省遗传资源势在必行。本研究将虹鱼精原细胞移植到新孵化的不育三倍体马苏鲑鱼体内。移植的鲑鱼精原细胞在雄性和雌性受体中分别进行了精子发生和卵子发生。在移植后2年，三倍体鲑鱼受体只产生鲑鱼精子和卵。利用这些鲑鱼作为亲本，我们成功地培育出了捐赠者衍生的鲑鱼后代。因此，通过将冷冻保存的精原细胞移植到无菌异种受体中，我们可以产生受威胁物种的个体。
Many salmonids have become at risk of extinction. For teleosts whose eggs cannot be cryopreserved, developing techniques other than egg cryopreservation to save genetic resources is imperative. In this study, spermatogonia from rainbow trout were intraperitoneally transplanted into newly hatched sterile triploid masu salmon. Transplanted trout spermatogonia underwent spermatogenesis and oogenesis in male and female recipients, respectively. At 2 years after transplantation, triploid salmon recipients only produced trout sperm and eggs. With use of these salmon as parents, we successfully produced only donor-derived trout offspring. Thus, by transplanting cryopreserved spermatogonia into sterile xenogeneic recipients, we can generate individuals of a threatened species.