Middle East Respiratory Syndrome Coronavirus Antibody Reactors Among Camels in Dubai, United Arab Emirates, in 2005
Middle East Respiratory Syndrome Coronavirus Antibody Reactors Among Camels in Dubai, United Arab Emirates, in 2005
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DOI：
10.1111/tbed.12212
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发表时间：
2014-04-01
影响因子：
4.3
通讯作者：
Wernery, U.
中科院分区：
文献类型：
--
作者：
Alexandersen, S.;Kobinger, G. P.;Wernery, U.
我们使用低起始稀释度，对 2005 年 2 月/4 月至 10 月在迪拜收集的单峰骆驼、绵羊和马的连续血清样本以及 2000 年至 2001 年加拿大和美国之间进行进出口测试的单峰骆驼进行了测试。使用标准的中东呼吸综合征冠状病毒 (MERS-CoV) 中和试验，三只羊和三匹马的系列血清均为阴性，而来自迪拜的 11 头单峰骆驼中的 9 头的血清抗体呈阳性，这得到了 MERS-CoV 重组部分刺突蛋白抗体 ELISA 中类似结果的支持。两只呈阴性的迪拜骆驼都是单峰骆驼，在研究的 5 个月内一直呈阴性。来自美国和加拿大的六只单峰骆驼样本在两次检测中均呈阴性。这些结果支持了最近的发现，即中东呼吸综合征冠状病毒或密切相关的病毒感染并不是中东骆驼中新出现的现象。因此，中东呼吸综合征冠状病毒在人与动物界面上的相互作用可能已经持续了数年，也许很多年，由此推断，除非病毒的显着进化允许加速感染和在人群中传播的可能性，否则大范围流行的可能性可能较小。
We tested, using a low starting dilution, sequential serum samples from dromedary camels, sheep and horses collected in Dubai from February/April to October of 2005 and from dromedary camels for export/import testing between Canada and USA in 2000-2001. Using a standard Middle East respiratory syndrome coronavirus (MERS-CoV) neutralization test, serial sera from three sheep and three horses were all negative while sera from 9 of 11 dromedary camels from Dubai were positive for antibodies supported by similar results in a MERS-CoV recombinant partial spike protein antibody ELISA. The two negative Dubai camels were both dromedary calves and remained negative over the 5months studied. The six dromedary samples from USA and Canada were negative in both tests. These results support the recent findings that infection with MERS-CoV or a closely related virus is not a new occurrence in camels in the Middle East. Therefore, interactions of MERS-CoV at the human-animal interface may have been ongoing for several, perhaps many, years and by inference, a widespread pandemic may be less likely unless significant evolution of the virus allow accelerated infection and spread potential in the human population.