Influence of glucose fermentation on CO2 assimilation to acetate in homoacetogen Blautia coccoides GA-1
Influence of glucose fermentation on CO2 assimilation to acetate in homoacetogen Blautia coccoides GA-1
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DOI：
10.1007/s10295-015-1646-1
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发表时间：
2015-09-01
影响因子：
3.4
通讯作者：
Meng, Jia
中科院分区：
文献类型：
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作者：
Liu, Chong;Li, Jianzheng;Meng, Jia
葡萄糖发酵影响同型乙酸菌中二氧化碳同化为乙酸。研究Blautia coccoides是为了更好地了解混合营养培养物中同型产乙酸菌的代谢特征。以H-2/CO2作为唯一碳源的菌株的分批培养在培养240小时后达到了5.32g/g干细胞重的乙酸盐产量。当向培养物中添加葡萄糖时，自养代谢受到抑制：葡萄糖浓度越高，细菌的自养能力越低。高葡萄糖浓度抑制自养，可能是由于 Embden-Meyerhof-Parnas 途径和 Wood-Ljungdahl 碳固定途径之间对辅酶 A 的竞争、细胞碳合成和 CO2 还原的能量（三磷酸腺苷）分配，以及乙酸积累引起的低 pH 值。
Fermentation of glucose influences CO2 assimilation to acetate in homoacetogens. Blautia coccoides was investigated for a better understanding of the metabolic characteristics of homoacetogens in mixotrophic cultures. Batch cultures of the strain with H-2/CO2 as a sole carbon source reached an acetate yield of 5.32 g/g dry cell weight after 240 h of incubation. Autotrophic metabolism was inhibited as glucose was added into the culture: the higher the glucose concentration the lower the autotrophic ability of the bacterium. Autotrophy was inhibited by high glucose concentration, probably due to the competition for coenzyme A between the Embden-Meyerhof-Parnas pathway and the Wood-Ljungdahl carbon fixation pathway, the energy (adenosine triphosphate) allocation for synthesis of cell carbon and reduction of CO2, in combination with the low pH caused by the accumulation of acetate.