IL-8 released from human lung epithelial cells induced by cystic fibrosis pathogens Burkholderia cepacia complex affects the growth and intracellular survival of bacteria
IL-8 released from human lung epithelial cells induced by cystic fibrosis pathogens Burkholderia cepacia complex affects the growth and intracellular survival of bacteria
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DOI：
10.1016/j.ijmm.2010.06.005
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发表时间：
2011-01-01
影响因子：
4.1
通讯作者：
Callaghan, Maire
中科院分区：
文献类型：
--
作者：
Kaza, Seshu K.;McClean, Siobhan;Callaghan, Maire
洋葱伯克霍尔德菌复合体（Bcc）是一组革兰氏阴性肺部病原体，与囊性纤维化（CF）患者的危及生命的感染相关。气道上皮细胞在这些病原体的炎症反应的启动和调节中起着至关重要的作用。上皮细胞释放的白细胞介素（IL）-8对中性粒细胞是一种有效的化学引诱物。本研究的目的是比较IL-8在不同类型上皮细胞中对Bcc感染的反应，并研究IL-8对Bcc生长和细胞内存活的影响。A549细胞、肺泡上皮细胞系、Calu-3细胞、支气管下上皮细胞系、16 HBE 14 o-细胞和CFBE 41 o-细胞，它们是CFTR阳性和CFTR阴性支气管上皮细胞系，分别两个B。多噬菌体和2个B. cenocepacia菌株在12小时时均诱导显著的IL-8应答，并且在24小时时在所有细胞系中进一步增加。此外，来自Calu-3和A549细胞的IL-8水平是16 HBE 140-或CFBE 410-细胞的约3倍。在检查的2个细胞系（16 HBE 14 o-和CFBE 41 o-）中，B.流行株新洋葱LMG 16656（J2315）诱导IL-8水平升高（P
Burkholderia cepacia complex (Bcc) is a group of Gram-negative pulmonary pathogens associated with life-threatening infections in patients with cystic fibrosis (CF). The airway epithelium plays a crucial role in the initiation and modulation of inflammatory responses to these pathogens. Interleukin (IL)-8 released from epithelial cells is a potent chemoattractant for neutrophils. The aims of this study were to compare the IL-8 response to Bcc infection in different epithelial cell types and to investigate the impact of IL-8 on Bcc growth and intracellular survival.To compare epithelial cell IL-8 responses, 4 human epithelial cell lines were used in the study; A549 cells, an alveolar epithelial cell line, Calu-3 cells, a sub-bronchial epithelial cell line, 16HBE14o-cells, and CFBE41o-cells, which are CFTR-positive and CFTR-negative bronchial epithelial cell lines, respectively. Two B. multivorans and 2 B. cenocepacia strains all induced a significant IL-8 response by 12 h and further increased in all cell lines at 24 h. Furthermore, the levels of IL-8 from Calu-3 and A549 cells were approximately 3 times that of 16HBE14o- or CFBE41o- cells. In 2 of the cell lines examined (16HBE14o- and CFBE41o-), B. cenocepacia LMG 16656 (J2315), an epidemic strain, induced greater levels of IL-8 (P