Identification of repertoires of surface antigens on leukemias using an antibody microarray
Identification of repertoires of surface antigens on leukemias using an antibody microarray
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DOI：
10.1002/pmic.200300599
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发表时间：
2003-11-01
期刊：
影响因子：
3.4
通讯作者：
Christopherson, RI
中科院分区：
文献类型：
--
作者：
Belov, L;Huang, P;Christopherson, RI
我们之前已经描述了一种分化簇(CD)抗体微阵列，它能够同时检测白细胞上的60多种CD抗原。这个过程不需要蛋白质纯化或标记，也不需要二次检测系统。整个细胞被一个由10个NL抗体点组成的微阵列捕获，该微阵列固定在显微镜载玻片上的硝酸纤维素膜上。不同的白血病或淋巴瘤观察到不同的细胞结合模式。这些血液系统恶性肿瘤起源于T或B淋巴细胞的前体细胞，或造血系的髓系细胞。患者外周血白细胞的斑点图案与正常人外周血白细胞的不同。这项微阵列技术最近经历了一些改进。微阵列现在包含更多的CD抗体，用于成像点模式的扫描仪和用于数据分析的软件提供了足以诊断常见白血病的广泛的免疫表型。目前正在评估这项技术在诊断白血病方面的作用，同时也在使用常规诊断标准。
We have previously described a microarray of cluster of differentiation (CD) antibodies that enables concurrent determination of more than 60 CD antigens on leukocytes. This procedure does not require protein purification or labeling, or a secondary detection system. Whole cells are captured by a microarray of 10 nL antibody dots immobilized on a nitrocellulose film on a microscope slide. Distinct patterns of cell binding are observed for different leukemias or lymphomas. These haematological malignancies arise from precursor cells of T- or B-lymphocytic, or myeloid lineages of hematopoiesis. The dot patterns obtained from patients are distinct from those of peripheral blood leukocytes from normal subjects. This microarray technology has recently undergone a number of refinements. The microarray now contains more CD antibodies, and a scanner for imaging dot patterns and software for data analysis provide an extensive immunophenotype sufficient for diagnosis of common leukemias. The technology is being evaluated for diagnosis of leukemias with parallel use of conventional diagnostic criteria.