Immunofluorescence analysis of the basement membrane zone components in human anagen hair follicles
Immunofluorescence analysis of the basement membrane zone components in human anagen hair follicles
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DOI：
10.1034/j.1600-0625.2002.120402.x
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发表时间：
2003-08
影响因子：
3.6
通讯作者：
S. Joubeh;O. Mori;K. Owaribe;T. Hashimoto
中科院分区：
文献类型：
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作者：
S. Joubeh;O. Mori;K. Owaribe;T. Hashimoto
摘要：毛囊间表皮基底膜区（BMZ）的超微结构和组成成分已得到很好的表征。然而，毛乳头和周围的毛囊上皮细胞之间的连接，以及毛囊外的结缔组织和上皮细胞之间的连接知之甚少。在本研究中，免疫荧光法用于确定各种BMZ组分的表达，特别是plectin、BP230、BP180、α6β4整合素、层粘连蛋白5和VII型胶原在人头皮生长期毛囊中的表达。检测的所有BMZ组分显示基本相同的免疫荧光染色模式。具体地说，毛囊上部的染色显示所有BMZ组分的表达，其标记强度与毛囊间表皮中发现的标记强度相似。然而，毛囊下部的染色明显不同：所有BMZ组分的染色强度均逐渐降低。特别是在毛球外，线性染色减少，甚至在某些区域不连续。最后，毛乳头和上皮细胞之间的毛球内，有一个强烈的免疫反应，所有的BMZ成分，除了BP 230，这是完全阴性。本研究还证实了以前报道的超微结构发现，半桥粒是不明显的毛球的外部BMZ，也没有在毛乳头交界处。相反，在致密层和毛球外的相邻上皮中均观察到特殊的混浊物质。两者合计，所有的BMZ组件外的毛球，以及在毛乳头交界处的BP 230的完全不存在的表达减少，似乎是负责在这些地区的半桥粒的不完整的超微结构。此外，本研究的结果使我们推测，与毛囊上部所示的永久区域中的表达相比，毛囊瞬时区域中的毛囊内外BMZ组分的表达显著降低。当毛囊在退行期向上移动或在生长期向下移动时，半桥粒的完整结构可以使上部稳定于周围的结缔组织，而不完整的半桥粒可以促进过渡区的移动。
Abstract: The ultrastructure and constituent components of the basement membrane zone (BMZ) of the interfollicular epidermis have been well characterized. However, little is known about the junctions between dermal papilla and the surrounding epithelial cells of the hair bulb, as well as those junctions between connective tissues and epithelial cells outside the hair follicle. In the present study, immunofluorescence was used to determine the expression of various BMZ components, particularly plectin, BP230, BP180, α6β4 integrin, laminin 5 and type VII collagen, in anagen hair follicles from human scalp. All the BMZ components examined showed essentially the same immunofluorescence staining pattern. Specifically, staining of the upper portion of the hair follicle demonstrated expression of all BMZ components with a labeling intensity similar to that found in the interfollicular epidermis. Staining in the lower portion of the hair follicle, however, was markedly different: all the BMZ components showed a gradual decrease in staining intensity. Particularly, outside the hair bulb, the linear staining was diminished and even discontinuous in some areas. Finally, between dermal papilla and epithelial cells inside the hair bulb, there was a strong immunoreactivity for all the BMZ components except for BP230, which was completely negative. The present study also confirmed a previous reported ultrastructural finding that hemidesmosomes are not apparent in the hair bulb's exterior BMZ nor in the dermal papilla junctions. Instead, peculiar cloudy materials were seen in both the lamina densa and the adjacent epithelium outside the hair bulb. Taken together, the diminished expression of all the BMZ components outside the hair bulb, as well as the complete absence of BP230 at the dermal papilla junction, seem to be responsible for the incomplete ultrastructure of hemidesmosomes in these regions. Furthermore, the results in the present study led us to speculate that the expression of BMZ components inside and outside the hair bulb are markedly decreased in the transient regions of the hair follicle as compared with their expression in the permanent region, signified by the upper portion of the hair follicle. When the hair follicle moves upward in catagen or downward in anagen, the complete structure of the hemidesmosome may stabilize the upper portion to the surrounding connective tissues, while the incomplete hemidesmosome may facilitate the movement of the transient region.