The expression of iron homeostasis-related genes during rice germination
The expression of iron homeostasis-related genes during rice germination
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DOI：
10.1007/s11103-007-9132-4
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发表时间：
2007-05-01
影响因子：
5.1
通讯作者：
Nishizawa, Naoko K.
中科院分区：
文献类型：
--
作者：
Nozoye, Tomoko;Inoue, Haruhiko;Nishizawa, Naoko K.
为了表征种子萌发早期阶段的Fe稳态，进行了微阵列分析。从完全成熟的种子或播种后1-3天收获的种子中提取的mRNA与含有约22，000个cDNA寡核苷酸探针的水稻微阵列杂交。许多铁缺乏诱导的基因强烈表达在整个早期种子萌发。这些结果表明，铁的需求是非常高的发芽过程中。在缺铁的条件下，水稻产生并分泌一种金属阳离子螯合剂，称为脱氧麦根酸（DMA），以从土壤中获得铁。此外，DMA及其中间体烟酰胺（NA）被认为参与了铁在水稻中的长距离运输。利用启动子-β-葡萄糖醛酸酶（GUS）分析，我们研究了在种子萌发过程中的表达模式的铁缺乏诱导基因OsNAS 1，OsNAS 2，OsNAS 3，OsNAAT 1，和OsDMAS 1，这些基因编码的酶参与DMA的生物合成，和转运基因OsYSL 2和OsIRT 1，这是参与铁的运输。所有这些基因都在胚根伸出之前的萌发种子中表达。这些结果表明，发芽过程中产生DMA和NA并参与Fe的运输。
To characterize Fe homeostasis during the early stages of seed germination, a microarray analysis was performed. mRNAs extracted from fully mature seeds or seeds harvested 1-3 days after sowing were hybridized to a rice microarray containing approximately 22,000 cDNA oligo probes. Many Fe deficiency-inducible genes were strongly expressed throughout early seed germination. These results suggest that the demand for Fe is extremely high during germination. Under Fe-deficient conditions, rice produces and secretes a metal-cation chelator called deoxymugineic acid (DMA) to acquire Fe from the soil. In addition, DMA and its intermediate nicotianamine (NA) are thought to be involved in long distance Fe transport in rice. Using promoter-beta-glucuronidase (GUS) analysis, we investigated the expression patterns during seed germination of the Fe deficiency-inducible genes OsNAS1, OsNAS2, OsNAS3, OsNAAT1, and OsDMAS1, which encode enzymes that participate in the biosynthesis of DMA, and the transporter genes OsYSL2 and OsIRT1, which are involved in Fe transport. All of these genes were expressed in germinating seeds prior to protrusion of the radicle. These results suggest that DMA and NA are produced and involved in Fe transport during germination.