Susceptibility of Spodoptera frugiperda (Lepidoptera: Noctuidae) Strains From Central Colombia to Two Insecticides, Methomyl and Lambda-Cyhalothrin: A Study of the Genetic Basis of Resistance
Susceptibility of Spodoptera frugiperda (Lepidoptera: Noctuidae) Strains From Central Colombia to Two Insecticides, Methomyl and Lambda-Cyhalothrin: A Study of the Genetic Basis of Resistance
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DOI：
10.1603/ec11079
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发表时间：
2011-10-01
影响因子：
2.2
通讯作者：
Saldamando-Benjumea, C. I.
中科院分区：
文献类型：
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作者：
Rios-Diez, J. D.;Saldamando-Benjumea, C. I.
夜蛾（鳞翅目：夜蛾科）是西半球几种农作物的重要害虫。这种昆虫在遗传上分化为两个与寄主相关的种群：玉米（Zea mays L.）和水稻（Oryza sativa L.）品系。玉米菌株也见于棉花（Gossypium hirsutum L.）和高粱，而水稻菌株见于百慕大草（Cynodon dactylon (L.)）。珀耳斯。和小米。在美国和巴西，用玉米、水稻、百慕大草和小米的品系来评估这两种品系对各种杀虫剂的抗性，发现玉米品系比水稻品系更具抗性。然而，在这些研究中，幼虫没有基因分型。在哥伦比亚，秋粘虫的基因分型是必要的，因为这种水稻菌株也可以在玉米地里发现。本研究从哥伦比亚中部Tolima地区的玉米和稻田中收集幼虫，对其进行基因分型，并对灭多威和高效氯氰菊酯进行抗性评价。我们发现，水稻品系对灭多威的抗性与玉米品系相比没有显著差异，但对高效氯氰菊酯的耐受性更快。灭多威对各代雌蜂卵活力也有显著影响。高效氯氟氰菊酯的抗性实现遗传力（0.23 ~ 0.42）高于灭多威（0.04 ~ 0.14）。抗性增加10倍所需的世代数，灭多威约为11.5代，高效氯氰菊酯约为6.5代。最后，对这两种杀虫剂的抗性的遗传基础涉及很少的隐性常染色体基因。结果表明，杀多威对秋粘虫的防治效果优于蓝素。
Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) is an important pest of several crops in the western hemisphere. This insect has genetically differentiated into two host-associated populations: the corn (Zea mays L.) and the rice (Oryza sativa L.) strains. The corn strain also is found in cotton (Gossypium hirsutum L.) and sorghum and the rice strain in Bermuda grass [Cynodon dactylon (L.) Pers.] and millet. In the United States and Brazil, lines from corn, rice, Bermuda grass, and millet were used to evaluate the resistance of both strains to various insecticides, and found that the corn strain is more resistant than the rice strain. However, in these studies the larvae were not genotyped. In Colombia, genotyping of fall armyworm is necessary because the rice strain also can be found in corn fields. In this work, collected larvae from corn and rice fields from Tolima (central Colombia) were genotyped and evaluated for the resistance to methomyl and lambdacyhalothrin. We found that the rice strain does not significantly differ in resistance to methomyl compared with the corn strain but it develops tolerance more rapidly to lambda-cyhalothrin. The eggs viability of treated females also was significantly affected by methomyl on each generation. The realized heritability of resistance was higher for lambda-cyhalothrin (0.23-0.42) than for methomyl (0.04-0.14). The number of generations needed for 10-fold increase in resistance is approximate to 11.5 generations for methomyl and 6.5 for lambda-cyhalothrin. Finally, the genetic basis of resistance to both insecticides involves few recessive autosomal genes. The results obtained here suggest that methomyl is a better option than lambda-cyalothin to control fall armyworm.