Media dependence of translational mutant phenotype
Media dependence of translational mutant phenotype
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DOI：
10.1111/j.1574-6968.1988.tb03189.x
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发表时间：
1988-12
影响因子：
2.1
通讯作者：
R. Mikkola;C. Kurland
中科院分区：
文献类型：
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作者：
R. Mikkola;C. Kurland
我们测量了大肠杆菌的一些核糖体突变体在不同生长培养基中的生长速率。该突变体是 rpsL 中的链霉素抗性（SmR）突变体； rpsL 中的部分链霉素依赖性 (SmP) 突变； rpsD 中的核糖体模糊突变体 (ram)； rpsE 中的公羊突变体以及 tRNA 修饰缺陷的突变体 mia A。数据表明，所有突变体的生长速率在贫乏培养基中受到的抑制程度低于在丰富培养基中的抑制程度。每个突变体的翻译率和无义抑制水平在富培养基和贫培养基中没有显着差异，这表明核糖体突变体表型在不同的生长条件下得以维持。这些结果表明，翻译机制改变的突变体的生长抑制程度取决于生长条件。此外，数据表明，细菌能够在生理上补偿此类突变体的生长效率损失，特别是在生长条件不佳的情况下。
We have measured the growth rates of some ribosomal mutants of Escherichia coli in different growth media. The mutants are a streptomycin resistant (SmR) mutation in rpsL; a partially streptomycin dependent (SmP) mutation in rpsL; a ribosome ambiguity mutant (ram) in rpsD; a ram mutant in rpsE as well as a mutant defective in tRNA modification, mia A. The data show that the growth rates of all mutants are less inhibited in poor media than they are in rich ones. The translation rates and nonsense suppression levels for each mutant are not significantly different in rich and poor media, which shows that the ribosomal mutant phenotypes are maintained under different growth conditions. These results suggest that the degree of growth inhibition for mutants with altered translation machinery is dependent on the growth conditions. In addition, the data suggest that bacteria are able to physiologically compensate for the loss of growth efficiency in such mutants, particularly, under poor growth conditions.