Localization capability and limitation of electron-multiplying charge-coupled, scientific complementary metal-oxide semiconductor, and charge-coupled devices for superresolution imaging
Localization capability and limitation of electron-multiplying charge-coupled, scientific complementary metal-oxide semiconductor, and charge-coupled devices for superresolution imaging
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用于超分辨率成像的电子倍增电荷耦合、科学互补金属氧化物半导体和电荷耦合器件的定位能力和局限性
DOI：
10.1117/1.3505017
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发表时间：
2010-11-01
影响因子：
3.5
通讯作者：
Huang, Zhen-Li
中科院分区：
文献类型：
--
作者：
Quan, Tingwei;Zeng, Shaoqun;Huang, Zhen-Li
单个荧光分子的定位在许多用于在细胞和亚细胞水平上可视化生物结构的超分辨率成像技术中是必需的。弱光探测器的定位能力和局限性对于这一目的至关重要。我们提出了一个最新的评估三个典型的低光探测器的性能，包括一个流行的电子倍增CCD（EMCCD），一个新开发的科学CMOS（sCMOS），和一个代表性的冷却CCD，超分辨率成像。我们发现，在一些实验条件下，sCMOS相机表现出竞争力，甚至更好的性能比EMCCD相机，这一直被认为是超分辨率成像领域的首选探测器。(C)2010年，美国光学仪器工程师学会（Society of Photo-Optical Instrumentation Engineers）[DOI 10.1117/1.3505017]
Localization of a single fluorescent molecule is required in a number of superresolution imaging techniques for visualizing biological structures at cellular and subcellular levels. The localization capability and limitation of low-light detectors are critical for such a purpose. We present an updated evaluation on the performance of three typical low-light detectors, including a popular electron-multiplying CCD (EMCCD), a newly developed scientific CMOS (sCMOS), and a representative cooled CCD, for superresolution imaging. We find that under some experimental accessible conditions, the sCMOS camera shows a competitive and even better performance than the EMCCD camera, which has long been considered the detector of choice in the field of superresolution imaging. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3505017]