Antagonism between germ cell-less and Torso receptor regulates transcriptional quiescence underlying germline/soma distinction.
Antagonism between germ cell-less and Torso receptor regulates transcriptional quiescence underlying germline/soma distinction.
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DOI：
10.7554/elife.54346
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发表时间：
2021-01-18
期刊：
影响因子：
7.7
通讯作者：
Deshpande G
中科院分区：
文献类型：
--
作者：
Colonnetta MM;Lym LR;Wilkins L;Kappes G;Castro EA;Ryder PV;Schedl P;Lerit DA;Deshpande G
转录静止是胚胎原始生殖细胞（PGCs）区别于其体细胞邻居的一个进化上保守的特征。在黑腹果蝇中，来自胚胎的PGCs由于无生殖细胞（gcl）而受到母体损害，错误表达体细胞基因，可能导致PGC丢失。最近的研究记录了在末端图案化决定簇（躯干受体）的蛋白水解降解期间对Gcl的需要。在这里，我们证明了女性命运的体细胞决定因素，性致死（Sxl），是一个生物学相关的Gcl的转录靶点。强调由Gcl介导的转录沉默的意义，躯干（torsoDeg）的抗降解形式的异位表达可以激活PGCs中的Sxl转录，而同时失去躯干样（tsl）恢复gcl PGCs的静止状态。有趣的是，像gcl突变体一样，来自在种系中表达torsoDeg的母体的胚胎显示极质RNA的异常扩散，表明Gcl和Torso之间的相互拮抗作用确保了种系/索马区别背后的种质的受控释放。
Transcriptional quiescence, an evolutionarily conserved trait, distinguishes the embryonic primordial germ cells (PGCs) from their somatic neighbors. In Drosophila melanogaster, PGCs from embryos maternally compromised for germ cell-less (gcl) misexpress somatic genes, possibly resulting in PGC loss. Recent studies documented a requirement for Gcl during proteolytic degradation of the terminal patterning determinant, Torso receptor. Here we demonstrate that the somatic determinant of female fate, Sex-lethal (Sxl), is a biologically relevant transcriptional target of Gcl. Underscoring the significance of transcriptional silencing mediated by Gcl, ectopic expression of a degradation-resistant form of Torso (torsoDeg) can activate Sxl transcription in PGCs, whereas simultaneous loss of torso-like (tsl) reinstates the quiescent status of gcl PGCs. Intriguingly, like gcl mutants, embryos derived from mothers expressing torsoDeg in the germline display aberrant spreading of pole plasm RNAs, suggesting that mutual antagonism between Gcl and Torso ensures the controlled release of germ-plasm underlying the germline/soma distinction.