Sodium Glucocorticoid Hemisuccinate Analysis in Biological Media Using Capillary Zone Electrophoresis with Bubble Cell
Sodium Glucocorticoid Hemisuccinate Analysis in Biological Media Using Capillary Zone Electrophoresis with Bubble Cell
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使用泡池毛细管区带电泳分析生物介质中的糖皮质激素半琥珀酸钠
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
R. Neubert
中科院分区：
文献类型：
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作者：
Y. Mrestani;S. Claussen;R. Neubert
建立了一种带灵敏池（气泡池）的毛细管区带电泳（CZE）方法，用于测定生物介质（血浆、尿液）中糖皮质激素半琥珀酸钠（Urbason® solubile forte 1000、Hydrocortison 100 Rotexmedica®、Prednisolut 100®）。将尿液中的样品在水中稀释（1：1 v/v），并注入仪器中，无需进一步制备样品。还定量分析了血浆中的制剂。在这里，有必要沉淀蛋白质组分。用乙腈（1：3 v/v）处理，效果良好。目前的结果表明，使用毛细管气泡池的调查导致了约9倍的检测限的改善相比，标准毛细管。使用泼尼松龙半琥珀酸盐浓度在2.5和500 μg.mL-1之间的校准曲线分析生物培养基中的样品。测定了迁移时间和峰面积的检测限和相对标准偏差。
A capillary zone electrophoresis (CZE) method with sensitivity cell (bubble cell) has been developed for the determination of sodium glucocorticoid hemisuccinates (Urbason® solubile forte 1000, Hydrocortison 100 Rotexmedica®, Prednisolut 100®) in biological media (plasma, urine). The samples in urine were diluted (1:1 v/v) in water and were injected into the apparatus without any further sample preparation. In plasma the formulations were also analyzed quantitatively. Here it was necessary to precipitate the protein component. Good results were achieved by treating with acetonitrile (1:3 v/v). The present results have shown that the investigation using a capillary with bubble cell led to an approximately 9-fold improvement of the detection limit compared to standard capillaries. The samples in the biological media were analyzed using a calibration curve for prednisolone hemisuccinate concentrations between 2.5 and 500 μg.mL−1. The detection limit and the relative standard deviation of the migration times and of the peak areas were determined.