Iminocoumarin based fluorophores: Indispensable scaffolds for rapid, selective and sensitive detection of thiophenol
Iminocoumarin based fluorophores: Indispensable scaffolds for rapid, selective and sensitive detection of thiophenol
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DOI：
10.1016/j.dyepig.2014.02.001
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发表时间：
2014-07-01
影响因子：
4.5
通讯作者：
Talukdar, Pinaki
中科院分区：
文献类型：
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作者：
Kand, Dnyaneshwar;Mandal, Prashant Sahebrao;Talukdar, Pinaki
根据苯硫酚的反应机理，设计了检测苯硫酚的关-开荧光探针，保证了传感过程的快速性。通过理论计算验证了2，4-二硝基苯磺酰基亚胺香豆素荧光团探针的非荧光态。这些探针与苯硫酚反应释放出强荧光的亚胺香豆素物种，具有良好的响应时间。这些结果与已报道的探针相比，证实了较高的pK（aH）值的亚氨基香豆素的亚氨基氮的快速传感过程是重要的。这些探针的反应性仅限于苯硫酚，没有观察到与脂肪族硫醇的反应。苯并噻唑取代的探针与苯硫酚反应后显示高达260倍的荧光增强。通过探针感测分析物的特征在于颜色从红色变为黄色并且出现绿色荧光。该探针的检测限也高达6.9 × 10（-9）M。(C)2014爱思唯尔有限公司版权所有。
Off-on fluorescence probes for the detection of thiophenol were designed based on the reaction mechanism to ensure rapid sensing process. 2,4-Dinitrophenyisulfonyl based probes with iminocoumarin fluorophores were validated by theoretical calculations for their off-fluorescence states. Reaction of these probes with thiophenol released strong fluorescent iminocoumarin species with good response times. These results, upon comparing with reported probes, confirmed that higher pK(aH) values of the imino nitrogen of iminocoumarins are important for rapid sensing process. Reactivity of these probes was limited to only thiophenol and no reaction was observed with aliphatic thiols. The benzothiazole substituted probe displayed up to 260-fold fluorescence enhancement upon reaction with thiophenol. Sensing of the analyte by the probe was characterized by change in color from red to yellow and with appearance of the green fluorescence. A detection limit up to 6.9 x 10(-9) M was also determined for this probe. (C) 2014 Elsevier Ltd. All rights reserved.