One-pot preparation of a sulfamethoxazole functionalized affinity monolithic column for selective isolation and purification of trypsin.
One-pot preparation of a sulfamethoxazole functionalized affinity monolithic column for selective isolation and purification of trypsin.
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DOI：
10.1016/j.chroma.2015.04.046
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发表时间：
2015-06
期刊：
影响因子：
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通讯作者：
Yuan Xiao;Jialiang Guo;Danni Ran;Qi-Rong Duan;J. Crommen;Zhengjin Jiang
中科院分区：
文献类型：
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作者：
Yuan Xiao;Jialiang Guo;Danni Ran;Qi-Rong Duan;J. Crommen;Zhengjin Jiang
采用“一锅法”制备磺胺类药物功能化整体柱。以磺胺甲基异恶唑（SMX）或磺胺（SAA）为药物配体，甲基丙烯酸缩水甘油酯（GMA）为单体，二甲基丙烯酸乙二醇酯（EDMA）为交联剂，采用一步原位聚合法，在100 μm内径内制备了两种新型的SA固定化甲基丙烯酸酯整体柱，即poly（GMA-SMX-co-EDMA）和poly（GMA-SAA-co-EDMA）。在优化的聚合条件下的毛细管。采用元素分析、扫描电镜和微型高效液相色谱等手段对所制备整体柱的理化性质和柱性能进行了表征。在优化的poly（GMA-SMX-co-EDMA）整体柱上对标准混合物和8种芳香酮等小分子物质进行了分离，获得了满意的柱透过率、分离效率和分离性能。值得注意的是，发现聚（GMA-SMX-共-EDMA）整料显示出对胰蛋白酶的选择性亲和力，而含有磺胺的聚（GMA-SAA-共-EDMA）整料根本不显示出这种亲和力。本研究不仅为胰蛋白酶的选择性分离和纯化提供了一种新的整体柱，而且还提供了通过一锅共聚策略容易地制备新型药物功能化甲基丙烯酸酯整体柱的可能性。
A facile and efficient “one-pot” copolymerization strategy was used for the preparation of sulfonamide drug (SA) functionalized monolithic columns. Two novel SA-immobilized methacrylate monolithic columns, i.e. poly(GMA-SMX-co-EDMA) and poly(GMA-SAA-co-EDMA) were prepared by one-potin situcopolymerization of the drug ligand (sulfamethoxazole (SMX) or sulfanilamide (SAA)), the monomer (glycidyl methacrylate, GMA) and the cross-linker (ethylene dimethacrylate, EDMA) within 100 μm i.d. capillaries under optimized polymerization conditions. The physicochemical properties and column performance of the fabricated monolithic columns were characterized by elemental analysis, scanning electron microscopy and micro-HPLC. Satisfactory column permeability, efficiency and separation performance were obtained on the optimized poly(GMA-SMX-co-EDMA) monolithic column for small molecules, such as a standard test mixture and eight aromatic ketones. Notably, it was found that the poly(GMA-SMX-co-EDMA) monolith showed a selective affinity to trypsin, while the poly(GMA-SAA-co-EDMA) monolith containing sulfanilamide did not exhibit such affinity at all. This research not only provides a novel monolith for the selective isolation and purification of trypsin, but it also offers the possibility to easily prepare novel drug functionalized methacrylate monoliths through a one-pot copolymerization strategy.