CHARACTERIZATION OF A PLASMID FROM HELICOBACTER-PYLORI ENCODING A REPLICATION PROTEIN COMMON TO PLASMIDS IN GRAM-POSITIVE BACTERIA
CHARACTERIZATION OF A PLASMID FROM HELICOBACTER-PYLORI ENCODING A REPLICATION PROTEIN COMMON TO PLASMIDS IN GRAM-POSITIVE BACTERIA
复制标题
DOI：
10.1111/j.1365-2958.1991.tb02084.x
复制
发表时间：
1991-10-01
影响因子：
3.6
通讯作者：
TABAQCHALI, S
中科院分区：
文献类型：
--
作者：
KLEANTHOUS, H;CLAYTON, CL;TABAQCHALI, S
从幽门螺杆菌（Helicobacter pylori，Hp）中分离到一个1.5kb的隐蔽质粒。低严格性杂交分析，使用该质粒作为DNA探针，揭示了与该物种中的其他质粒的碱基序列同源性。核苷酸序列分析鉴定了编码25 kDa推定多肽的开放阅读框。这种蛋白质表现出显着的氨基酸序列相似性的复制起始蛋白质中常见的小质粒内源性革兰氏阳性菌复制的“滚圈”机制。在该隐蔽质粒上发现了对应于复制起点共有序列的序列基序。这些质粒复制序列的DNA和寡核苷酸探针用于杂交分析，以鉴定其他H. pylori质粒。我们相信这是第一个从革兰氏阴性细菌中分离出的质粒，显示出革兰氏阳性细菌质粒的“滚环”组的复制决定簇特征。克隆的质粒将用于构建H.幽门。
A 1.5kb cryptic plasmid was isolated from Helicobacter pylori. Low-stringency hybridization analysis using this plasmid as a DNA probe revealed base sequence homology with other plasmids in this species. Nucleotide sequence analysis identified an open reading frame encoding a putative polypeptide of 25 kDa. This protein showed marked amino acid sequence similarity to replication-initiation proteins commonly found in small plasmids endogenous to Gram-positive bacteria which replicate by the 'rolling-circle' mechanism. Sequence motifs corresponding to the origins-of-replication consensus sequences were found on this cryptic plasmid. DNA and oligonucleotide probes to these plasmid replication sequences were used in hybridization analysis to identify similar sequences in other H. pylori plasmids. We believe this is the first plasmid isolated from a Gram-negative bacterium to show replication determinants characteristic of the 'rolling-circle' group of plasmids from Gram-positive bacteria. The cloned plasmid will be used to develop a shuttle-vector for H. pylori.