Untargeted Metabolome Analysis of Alcohol-Exposed Pregnancies Reveals Metabolite Differences That Are Associated with Infant Birth Outcomes.
Untargeted Metabolome Analysis of Alcohol-Exposed Pregnancies Reveals Metabolite Differences That Are Associated with Infant Birth Outcomes.
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DOI：
10.3390/nu14245367
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发表时间：
2022-12-17
期刊：
影响因子：
5.9
通讯作者：
Smith SM
中科院分区：
文献类型：
--
作者：
Hasken JM;de Vries MM;Marais AS;May PA;Parry CDH;Seedat S;Mooney SM;Smith SM
产前酒精暴露会导致后代生长缺陷，是神经发育障碍的主要原因。我们使用非靶向代谢组学来产生酒精如何损害胎儿发育的机制见解。在南非西开普省，招募了52名妊娠5-36周（平均18.5±6.5）的妇女，她们提供了手指穿刺的空腹血点，并进行了质谱分析。使用偏最小二乘判别分析（PLS-DA）对代谢组学数据进行分析，以确定与酒精暴露和婴儿出生结局相关的代谢物。在过去7天内饮酒的女性的代谢物特征包括鞘磷脂、胆固醇和孕烯醇酮减少，脂肪酸、酰基和氨基酰基肉毒碱和雄酮升高。利用PLS-DA，区分母系群体的前30个代谢物中有25个被酒精还原，中链游离脂肪酸和氧化糖衍生物的影响最大。另一项单独的正交pls - da分析发现了一组与婴儿身高、体重和头围相关的13种代谢物。这些包括单酰基甘油、甘油-3-磷酸和未识别的代谢物，它们的大多数相关性是阴性的，这意味着它们代表了对胎儿发育有不良后果的过程。
Prenatal alcohol exposure can produce offspring growth deficits and is a leading cause of neurodevelopmental disability. We used untargeted metabolomics to generate mechanistic insight into how alcohol impairs fetal development. In the Western Cape Province of South Africa, 52 women between gestational weeks 5–36 (mean 18.5 ± 6.5) were recruited, and they provided a finger-prick fasting bloodspot that underwent mass spectrometry. Metabolomic data were analyzed using partial least squares-discriminant analyses (PLS-DA) to identify metabolites that correlated with alcohol exposure and infant birth outcomes. Women who consumed alcohol in the past seven days were distinguished by a metabolite profile that included reduced sphingomyelins, cholesterol, and pregnenolones, and elevated fatty acids, acyl and amino acyl carnitines, and androsterones. Using PLS-DA, 25 of the top 30 metabolites differentiating maternal groups were reduced by alcohol with medium-chain free fatty acids and oxidized sugar derivatives having the greatest influence. A separate ortho-PLS-DA analysis identified a common set of 13 metabolites that were associated with infant length, weight, and head circumference. These included monoacylglycerols, glycerol-3-phosphate, and unidentified metabolites, and most of their associations were negative, implying they represent processes having adverse consequences for fetal development.
DOI：
10.1007/s11306-021-01773-8
发表时间：
2021-02-07
期刊：
Metabolomics : Official journal of the Metabolomic Society
影响因子：
--
作者：
Saini N;Virdee M;Helfrich KK;Kwan STC;Smith SM
通讯作者：
Smith SM
DOI：
10.1111/j.1530-0277.2011.01732.x
发表时间：
2012-08
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
Palmer JA;Poenitzsch AM;Smith SM;Conard KR;West PR;Cezar GG
通讯作者：
Cezar GG