Aminophosphate precursors for the synthesis of near-unity emitting InP quantum dots and their application in liver cancer diagnosis.
Aminophosphate precursors for the synthesis of near-unity emitting InP quantum dots and their application in liver cancer diagnosis.
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氨基磷酸酯的前体合成近乎统一的INP量子点及其在肝癌诊断中的应用。
DOI：
10.1002/exp.20220082
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发表时间：
2022-08
期刊：
影响因子：
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通讯作者：
Shen, Huaibin
中科院分区：
文献类型：
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作者：
Zhang, Yanbin;Lv, Yanbing;Li, Lin-Song;Zhao, Xue-Jie;Zhao, Mei-Xia;Shen, Huaibin
在体外诊断和生物成像应用中，InP量子点（QDs）是一种有前途的、环境友好的替代Cd基量子点。然而，它们较差的荧光性和稳定性严重限制了它们在生物学上的应用。本研究利用低成本、低毒的磷源合成了明亮（~ 100%）且稳定的InP基核/壳量子点，并通过壳工程制备了量子产率超过80%的InP基水性量子点。利用这些基于InP量子点的荧光探针，可以在1 - 1000 ng ml - 1的最宽分析范围内检测到α胎蛋白，检测限为0.58 ng ml - 1，使其成为迄今为止报道的性能最好的无重金属检测，可与最先进的基于Cd量子点的探针相比较。此外，高质量的InP量子点在肝癌细胞的特异性标记和活体小鼠的体内肿瘤靶向成像方面表现优异。总的来说，目前的工作证明了新型高质量无镉InP量子点在癌症诊断和图像引导手术中的巨大潜力。利用氨基磷酸盐前驱体制备了量子产率为100%的环境友好型InP核/壳量子点（QDs）。在体外诊断、肝癌细胞特异性标记和活体小鼠体内肿瘤靶向成像方面，甲胎蛋白的检测限与目前最先进的基于Cd - QDs的探针相当。
InP quantum dots (QDs) are a promising and environment‐friendly alternative to Cd‐based QDs for in vitro diagnostics and bioimaging applications. However, their poor fluorescence and stability severely limit their biological applications. Herein, we synthesize bright (∼100%) and stable InP‐based core/shell QDs by using cost‐effective and low‐toxic phosphorus source, and then aqueous InP QDs are prepared with quantum yield over 80% by shell engineering. The immunoassay of alpha‐fetoprotein can be detected in the widest analytical range of 1–1000 ng ml−1 and the limit of detection of 0.58 ng ml−1 by using those InP QDs‐based fluorescent probes, making it the best‐performing heavy metal‐free detection reported so far, comparable to state‐of‐the‐art Cd‐QDs‐based probes. Furthermore, the high‐quality aqueous InP QDs exhibit excellent performance in specific labeling of liver cancer cells and in vivo tumor‐targeted imaging of live mice. Overall, the present work demonstrates the great potential of novel high‐quality Cd‐free InP QDs in cancer diagnosis and image‐guided surgery. Environment‐friendly InP core/shell quantum dots (QDs) with quantum yield of ∼100% are prepared by aminophosphate precursors. The limit of detection of alpha‐fetoprotein for in vitro diagnostics, specific labeling of liver cancer cells, and in vivo tumor‐targeted imaging of live mice are comparable to that of the state‐of‐the‐art Cd‐QDs‐based probes.