Skeletal muscle triglyceride levels are inversely related to insulin action
Skeletal muscle triglyceride levels are inversely related to insulin action
复制标题
DOI：
10.2337/diabetes.46.6.983
复制
发表时间：
1997-06-01
期刊：
影响因子：
7.7
通讯作者：
Storlien, LH
中科院分区：
文献类型：
--
作者：
Pan, DA;Lillioja, S;Storlien, LH
在动物研究中，与骨骼肌相关的甘油三酯 (mTG) 含量增加与骨骼肌和全身胰岛素作用降低相关。这项研究的目的是在人类中测试这种关系。受试者为 38 名非糖尿病男性皮马印第安人（平均年龄 28 +/- 1 岁）。通过正常血糖钳评估生理（M）和超生理（MZ）胰岛素水平的胰岛素敏感性。在胰岛素给药之前和期间通过间接量热法测定脂质和碳水化合物的氧化。 mTG 是通过经皮活检获得的股外侧肌测定的。通过水下称重测量身体脂肪百分比（平均 29 +/- 1%，范围 14-44%）。在简单回归中，mTG（平均值 5.4 +/- 0.3 pmol/g，范围 1.3-1.9 mu mol/g）和 log(10)M（r = -0.53，P 小于或等于 0.001）、MZ（r = -0.44，P = 0.006）和非氧化葡萄糖处理（生理条件下 r = -0.48 和 -0.47）之间存在负相关关系。和超生理胰岛素水平，分别为 P = 0.005)，但不影响葡萄糖或脂质氧化。 mTG 与任何肥胖指标均无关。在多元回归中，胰岛素抵抗指标（log(10)M、MZ、log(10)[空腹胰岛素]）与 mTG 显着相关，独立于所有肥胖指标（体脂百分比、BMI、腰臀比）。反过来，所有肥胖指标均与独立于 mTG 的胰岛素抵抗指标相关。在这些关系中，肥胖测量值和 mTG 在胰岛素抵抗差异中所占的比例相似。结果表明，在该人群中，与动物模型一样，骨骼肌胰岛素敏感性受到当地甘油三酯供应以及远程储存库和循环脂质的强烈影响。 mTG 和胰岛素对骨骼肌糖原合成的作用之间关系的潜在机制可能是理解胰岛素抵抗的核心。
In animal studies, increased amounts of triglyceride associated with skeletal muscle (mTG) correlate with reduced skeletal muscle and whole body insulin action. The aim of this study was to test this relationship in humans. Subjects were 38 nondiabetic male Pima Indians (mean age 28 +/- 1 years). Insulin sensitivity at physiological (M) and supraphysiological (MZ) insulin levels was assessed by the euglycemic clamp. Lipid and carbohydrate oxidation were determined by indirect calorimetry before and during insulin administration. mTG was determined in vastus lateralis muscles obtained by percutaneous biopsy. Percentage of body fat (mean 29 +/- 1%, range 14-44%) was measured by underwater weighing. In simple regressions, negative relationships were found between mTG (mean 5.4 +/- 0.3 pmol/g, range 1.3-1.9 mu mol/g) and log(10)M (r = -0.53, P less than or equal to 0.001), MZ (r = -0.44, P = 0.006), and nonoxidative glucose disposal (r = -0.48 and -0.47 at physiological and supraphysiological insulin levels, respectively, both P = 0.005) but not glucose or lipid oxidation. mTG was not related to any measure of adiposity. In multiple regressions, measures of insulin resistance (log(10)M, MZ, log(10)[fasting insulin]) were significantly related to mTG independent of all measures of obesity (percentage of body fat, BMI, waist-to-thigh ratio). In turn, all measures of obesity were related to the insulin resistance measures independent of mTG. The obesity measures and mTG accounted for similar proportions of the variance in insulin resistance in these relationships. The results suggest that in this human population, as in animal models, skeletal muscle insulin sensitivity is strongly influenced by local supplies of triglycerides, as well as by remote depots and circulating lipids. The mechanism(s) underlying the relationship between mTG and insulin action on skeletal muscle glycogen synthesis may be central to an understanding of insulin resistance.