Plate-induced tumors of BALB/3T3 cells exhibiting foci of differentiation into pericytes, chondrocytes, and fibroblasts.
Plate-induced tumors of BALB/3T3 cells exhibiting foci of differentiation into pericytes, chondrocytes, and fibroblasts.
复制标题
BALB/3T3 细胞的板诱导肿瘤表现出分化为周细胞、软骨细胞和成纤维细胞的灶。
DOI：
10.1002/jss.400140212
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Scott,RE
中科院分区：
文献类型：
--
作者：
Boone,CW;Scott,RE
BALB/3 T3克隆A31小鼠胚胎细胞系已被许多研究者用作各种体外研究的模型“正常”“成纤维细胞”系。然而，已经证明这些细胞不是“正常的”，因为如果将3 × 104个细胞皮下植入贴在0.2 × 5 × 10-mm塑料板上的BALB/c小鼠，它们将在2-4个月内产生肿瘤。以前的研究也表明这些细胞不是成纤维细胞，因为它们产生的肿瘤具有血管内皮细胞而不是成纤维细胞的特征。我们现在报告，当检查通过将这些细胞附着在塑料板上植入而衍生的肿瘤时，BALB/3 T3（克隆A31）、BALB/3 T3-T（克隆A31细胞的前脂肪细胞亚克隆）和BALB/3 T3-T细胞的6个最近亚克隆显示出额外的分化模式。观察到分化为周细胞、软骨细胞和成纤维细胞。我们的结论是，BALB/3 T3克隆A31细胞系和相关系是多能间充质细胞，能够分化成多种细胞类型。
The BALB/3T3 clone A31 mouse embryo cell line has been used by many investigators as a model “normal” “fibroblast” line for a variety of in vitro studies. It has been shown, however, that these cells are not “normal” because they will produce tumors within 2–4 months if 3 × 104cells are implanted subcutaneously in BALB/c mice attached to 0.2 × 5 × 10‐mm plastic plates. Previous studies also suggested that these cells were not fibroblasts because they gave rise to tumors with the characteristics of vascular endothelium not fibroblasts. We now report that BALB/3T3 (clone A31), BALB/3T3‐T, a proadipocyte subclone of clone A31 cells, and six recent subclones of BALB/3T3‐T cells show additional differentiation patterns when tumors derived by implantation of these cells attached to plastic plates are examined. Differentiation into pericytes, chondrocytes, and fibroblasts was observed. We conclude that the BALB/3T3 clone A31 cell line and related lines are multipotent mesenchymal cells which are capable of differentiation into a variety of cell types.