Osteoblast-like properties of human periodontal ligament cells: an in vitro analysis
Osteoblast-like properties of human periodontal ligament cells: an in vitro analysis
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DOI：
10.1093/ejo/19.6.615
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发表时间：
1997-12-01
影响因子：
2.6
通讯作者：
Komposch, G
中科院分区：
文献类型：
--
作者：
Basdra, EK;Komposch, G
识别存在牙周韧带（PDL）内的细胞的生物学特性将有助于理解这些细胞在牙周韧带的各种功能中所起的作用，并将改善临床程序的成功，例如正畸牙齿运动。为此，对从人牙周分离的成纤维细胞进行了培养，并在组织化学和生物化学上相对于其假定的成骨细胞样性质进行了表征。组织化学上，培养的PDL成纤维细胞在碱性磷酸酶（ALP）上显示出强烈的染色。从生化上讲，随着时间的推移，培养物的基础ALP活性增加。用1个α，25-二羟基维生素D-3刺激后的ALP水平明显高于对照培养物的ALP。此外，针对骨钙素的免疫荧光（高度可靠的成骨细胞标记）非常阳性。细胞培养物的冯·科萨（Von Kossa）染色揭示了矿物状结节的形成。这些结果表明，人PDL成纤维细胞表现出与成骨细胞样细胞一致的体外表型特征，因此表明此类细胞有可能区分成骨细胞和/或胶质细胞。
Identifying the biological properties of the cells residing within the periodontal ligament (PDL) will help in understanding the role that these cells play in the various functions of the periodontal ligament, and will improve the success of clinical procedures such as orthodontic tooth movement. For this purpose, fibroblasts isolated from human periodontium were cultured and characterized both histochemically and biochemically with respect to their putative osteoblast-like properties.Histochemically, cultured PDL fibroblasts showed an intense staining for alkaline phosphatase (ALP). Biochemically, the basal ALP activity increased in culture over time. ALP levels after stimulation with 1 alpha,25-dihydroxyvitamin D-3 were significantly higher than those of control cultures. Moreover, immunofluorescence against osteocalcin (a highly reliable osteoblastic marker) was strongly positive. Von Kossa staining of the cell cultures revealed the formation of mineral-like nodules. These results indicate that human PDL fibroblasts exhibit in vitro phenotypic characteristics consistent with osteoblast-like cells, thus suggesting that such cells have the potential to differentiate into osteoblasts and/or cementoblasts.