Genome-Wide Identification and Characterization of the LRR-RLK Gene Family in Two Vernicia Species.
Genome-Wide Identification and Characterization of the LRR-RLK Gene Family in Two Vernicia Species.
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两种漆树属 LRR-RLK 基因家族的全基因组鉴定和表征
DOI：
10.1155/2015/823427
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发表时间：
2015
影响因子：
2.9
通讯作者：
Chen Y
中科院分区：
文献类型：
--
作者：
Zhu H;Wang Y;Yin H;Gao M;Zhang Q;Chen Y
富含亮氨酸重复序列受体样激酶（Leucine-rich repeat receptor-like kinases，LRR-RLKs）是植物中最大的一类RLKs，在病原菌应答和信号转导等重要生物学过程中发挥重要作用。迄今为止，大多数关于LRR-RLK的研究都是在模型植物上进行的。在这里，我们确定了236和230 LRR-RLKs在两个工业产油树：油桐和油桐，分别。序列比对分析表明，Vf/VmLRR-RLKs中RLK结构域的同源性（23.81%）大于LRR结构域的同源性（9.51%）。Vf/VmLRR-RLKs中LRR结构域的保守基序与已知的植物LRR共有序列匹配良好，但在倒数第三个氨基酸（W或L）处不同。系统发育分析表明，Vf/VmLRR-RLKs可分为16个亚支。我们表征了Vf/VmLRR-RLKs在各种组织类型（包括根、叶、花瓣和仁）中的表达谱。进一步的研究表明，Vf/VmLRR-RLK直向同源基因对枯萎病的响应主要表现为相似的表达模式，只有4对Vf/VmLRR-RLK直向同源基因表现出相反的表达趋势。这些结果代表了LRR-RLKs在两种工业油树中的广泛评价，并将有助于这些蛋白的进一步功能研究。
Leucine-rich repeat receptor-like kinases (LRR-RLKs) make up the largest group of RLKs in plants and play important roles in many key biological processes such as pathogen response and signal transduction. To date, most studies on LRR-RLKs have been conducted on model plants. Here, we identified 236 and 230 LRR-RLKs in two industrial oil-producing trees: Vernicia fordii and Vernicia montana, respectively. Sequence alignment analyses showed that the homology of the RLK domain (23.81%) was greater than that of the LRR domain (9.51%) among the Vf/VmLRR-RLKs. The conserved motif of the LRR domain in Vf/VmLRR-RLKs matched well the known plant LRR consensus sequence but differed at the third last amino acid (W or L). Phylogenetic analysis revealed that Vf/VmLRR-RLKs were grouped into 16 subclades. We characterized the expression profiles of Vf/VmLRR-RLKs in various tissue types including root, leaf, petal, and kernel. Further investigation revealed that Vf/VmLRR-RLK orthologous genes mainly showed similar expression patterns in response to tree wilt disease, except 4 pairs of Vf/VmLRR-RLKs that showed opposite expression trends. These results represent an extensive evaluation of LRR-RLKs in two industrial oil trees and will be useful for further functional studies on these proteins.