Human melanoma metastasis is inhibited following ex vivo treatment with an antisense oligonucleotide to protein kinase C-alpha.
Human melanoma metastasis is inhibited following ex vivo treatment with an antisense oligonucleotide to protein kinase C-alpha.
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DOI：
10.1016/s0304-3835(98)00052-4
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发表时间：
1998-06
期刊：
影响因子：
9.7
通讯作者：
J. Dennis;N. Dean;C. Bennett;J. Griffith;C. Lang;D. Welch
中科院分区：
文献类型：
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作者：
J. Dennis;N. Dean;C. Bennett;J. Griffith;C. Lang;D. Welch
为了确定 PKCα 表达的改变是否会影响人黑色素瘤细胞的转移潜力，在体外用特异性抑制 PKCα 表达的硫代磷酸酯反义寡脱氧核苷酸 (ODN) (ISIS-3521) 处理 C8161 细胞的复制培养物。对照C8161培养物用乱序序列ODN、阳离子脂质体处理或不处理。 Northern 印迹显示，与对照组相比，ISIS-3521 处理的细胞中 PKCα mRNA 受到 70% 的抑制。当将 ISIS-3521 处理的细胞静脉注射到无胸腺小鼠体内时，转移被抑制了 75%。这些结果表明PKCα表达在人类黑色素瘤转移的调节中很重要。
To determine whether alteration of PKCα expression would affect the metastatic potential of human melanoma cells, replicate cultures of C8161 cells were treated in vitro with a phosphorothioate antisense oligodeoxynucleotide (ODN) that specifically inhibits PKCα expression (ISIS-3521). Control C8161 cultures were treated with a scrambled sequence ODN, cationic liposomes or were left untreated. Northern blots demonstrated 70% inhibition of PKCα mRNA in ISIS-3521-treated cells compared to controls. Metastasis was suppressed by 75% when ISIS-3521-treated cells were injected intravenously into athymic mice. These results show that PKCα expression is important in the regulation of human melanoma metastasis.