High dietary fat and the development of osteoarthritis in a rabbit model
High dietary fat and the development of osteoarthritis in a rabbit model
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DOI：
10.1016/j.joca.2012.02.007
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发表时间：
2012-06-01
影响因子：
7
通讯作者：
Ehrlich, M. G.
中科院分区：
文献类型：
--
作者：
Brunner, A. M.;Henn, C. M.;Ehrlich, M. G.
目的：骨关节炎（OA）与肥胖有关，尽管这种关系尚不清楚。提出的肥胖症OA的病因包括对线不良关节的机械负荷和膳食脂肪的可能毒性。在本研究中测试的假设是，增加膳食脂肪的OA在两个错位和正常的关节，检测生化和组织学软骨marker.Method：83新西兰白色兔分为两个条件相关的OA：弯曲的膝盖和14%千卡与47.8%千卡的脂肪饮食。在整个实验过程中比较了家兔体重和膝关节角度。在28和38周，关节内的力量进行了测量，动物处死，膝关节软骨检查组织学变化，糖胺聚糖含量，35 S摄取，和聚集蛋白聚糖酶-1 expression.Results：有两种饮食之间的动物体重或关节内的力量没有差异。尽管饮食中的脂肪含量增加，但47.8%千卡脂肪饮食的动物没有增加多余的体重。代表性组织学显示高脂肪饮食动物中关节软骨的非典型剪切。47.8%千卡脂肪饮食的动物蛋白质合成抑制相比，14%千卡脂肪饮食：较低的糖胺聚糖含量和聚集蛋白聚糖酶-1在所有膝关节腔室的表达在这两个时间，和较低的35 S摄取在38 weeks.Conclusion：这些结果表明，饮食脂肪，独立于动物体重，结果改变软骨细胞功能。增加的膳食脂肪与体内兔软骨的变化有关，似乎是OA发展的危险因素。(C)2012年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: Osteoarthritis (OA) is associated with obesity, although this relationship remains unclear. Proposed etiologies of OA in obesity include mechanical loading of malaligned joints and possible toxicity of dietary fat. The hypothesis tested in the present study was that increased dietary fat worsens OA in both malaligned and normal joints, detected by biochemical and histological cartilage markers.Method: 83 New Zealand white rabbits were divided among two conditions related to OA: bowing of the knee and a 14% kcal vs 47.8% kcal fat diet. Rabbit weights and knee angles were compared throughout the experiment. At 28 and 38 weeks, intra-articular forces were measured, animals sacrificed, and knee cartilage examined for histological changes, glycosaminoglycan content, 35S uptake, and aggrecanase-1 expression.Results: There were no differences in animal weights or intra-articular forces between the two diets. Despite increased fat content in their diet, animals on the 47.8% kcal fat diet did not gain excess weight. Representative histology showed atypical shearing of articular cartilage among animals on the high fat diet. Animals on the 47.8% kcal fat diet had suppression of protein synthesis compared to the 14% kcal fat diet: lower glycosaminoglycan content and aggrecanase-1 expression in all knee compartments at both times, and lower 35S uptake at 38 weeks.Conclusion: These results suggest dietary fat, independent of animal weight, results in altered chondrocyte function. Increased dietary fat was associated with changes in rabbit cartilage in vivo and appears to be a risk factor for the development of OA. (C) 2012 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.