Suppression and acceleration of cell elongation by integration of xyloglucans in pea stem segments
Suppression and acceleration of cell elongation by integration of xyloglucans in pea stem segments
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DOI：
10.1073/pnas.132080299
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发表时间：
2002-06
影响因子：
11.1
通讯作者：
T. Takeda;Y. Furuta;T. Awano;K. Mizuno;Y. Mitsuishi;Takahisa Hayashi
中科院分区：
文献类型：
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作者：
T. Takeda;Y. Furuta;T. Awano;K. Mizuno;Y. Mitsuishi;Takahisa Hayashi
木葡聚糖是生长中的植物细胞壁中的一种关键聚合物。通过对豌豆茎段和剥去表皮的茎段进行实验，研究人员发现，在壁结合型木聚糖内转糖基化酶的作用下，木聚糖的整合抑制了细胞的伸长，而其片段低聚糖的整合则加速了细胞的伸长。整个木葡聚糖进入细胞壁，诱导皮层微管由横向向纵向重排；相反，低聚糖溶解了细胞壁上的木葡聚糖，维持了微管的横向方向。本文提出木葡聚糖代谢控制植物细胞的伸长。
Xyloglucan is a key polymer in the walls of growing plant cells. Using split pea stem segments and stem segments from which the epidermis had been peeled off, we demonstrate that the integration of xyloglucan mediated by the action of wall-bound xyloglucan endotransglycosylase suppressed cell elongation, whereas that of its fragment oligosaccharide accelerated it. Whole xyloglucan was incorporated into the cell wall and induced the rearrangement of cortical microtubules from transverse to longitudinal; in contrast, the oligosaccharide solubilized xyloglucan from the cell wall and maintained the microtubules in a transverse orientation. This paper proposes that xyloglucan metabolism controls the elongation of plant cells.