Comparative study of in vitro caries-like lesions and natural caries lesions at crown margins.
Comparative study of in vitro caries-like lesions and natural caries lesions at crown margins.
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DOI：
10.1111/j.1532-849x.2007.00220.x
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发表时间：
2007-11-01
期刊：
影响因子：
--
通讯作者：
Gaengler, Peter
中科院分区：
文献类型：
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作者：
Arnold, Wolfgang H;Sonkol, T;Gaengler, Peter
目的：冠边缘继发龋及预备技术的影响是临床上的主要问题。因此，本研究的目的是比较自然龋损在冠边缘与实验性龋损。材料和方法：五个提取的无龋的人磨牙恢复与金铸冠，然后覆盖蜡，留下一个3 × 3毫米的窗口在冠边缘。然后将这些牙齿在酸化凝胶（pH 4.7）中孵育50天。孵育后，将这些牙齿和其他五颗表现出自然龋齿病变的牙冠拔出的牙齿包埋在Technovit 9100中。采用偏光显微镜（PLM）、扫描电镜（SEM）结合能谱分析（EDX）对病变组织进行Ca、P、C元素定量分析。定量元素分析的结果进行统计学评价，使用非参数方差分析测试重复measurement.Results：PLM的实验病灶显示均匀的病变，没有透明区，或死道。自然龋损表现为脱矿区、半透明区和死道。定量元素分析显示，在自然和实验性龋损中，健康牙本质和脱矿牙本质中Ca、P和C的差异有统计学意义（p < 0.01）。结论：实验模型再现了继发性龋的脱矿模式，但不能模拟管周和管内矿化的重要牙本质反应。
PURPOSE: Secondary caries at crown margins and the influence of preparation techniques are major clinical problems. It was therefore the aim of this study to compare natural caries lesions at crown margins with experimental caries lesions.MATERIALS AND METHODS: Five extracted caries-free human molars were restored with gold cast crowns and afterwards covered with wax leaving a 3 x 3 mm window at the crown margin. These teeth were then incubated in acidified gel (pH 4.7) for 50 days. After incubation these teeth and five other crowned extracted teeth exhibiting natural caries lesions were embedded in Technovit 9100. Serial sections with a thickness of 80 microm were cut through the lesions and investigated with polarized light microscopy (PLM) and scanning electron microscopy (SEM) combined with energy dispersive X-ray (EDX) analysis for quantitative element analysis of Ca, P, and C. The results of the quantitative element analysis were statistically evaluated using the nonparametric ANOVA test for repeated measurements.RESULTS: PLM of the experimental lesions showed homogeneous lesions with no transparent zone, or dead tracts. The natural caries lesions exhibited a demineralized zone, translucent zone, and dead tracts. Quantitative element analysis showed a statistically significant difference (p < 0.01) of Ca, P, and C between sound dentin and demineralized dentin in natural and experimental caries lesions.CONCLUSION: The experimental model reproduces the demineralization pattern of secondary caries but does not simulate the vital dentin reactions of peritubular and intratubular mineralization.