Construction strategy for ratiometric fluorescent probe based on Janus silica nanoparticles as a platform toward intracellular pH detection.
Construction strategy for ratiometric fluorescent probe based on Janus silica nanoparticles as a platform toward intracellular pH detection.
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DOI：
10.1016/j.talanta.2019.06.021
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发表时间：
2019-12
期刊：
影响因子：
6.1
通讯作者：
Yang Xing;Ying Zhou;Li Fan;Yajuan Yang;Y. Zhang;Xu Deng;C. Dong;S. Shuang
中科院分区：
文献类型：
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作者：
Yang Xing;Ying Zhou;Li Fan;Yajuan Yang;Y. Zhang;Xu Deng;C. Dong;S. Shuang
在此，我们设计了一种基于Janus二氧化硅纳米颗粒（SiO2NPs）的新型比例荧光探针，其中异硫氰酸荧光素（FITC）和罗丹明B （RB）被不对称功能化，用于细胞内pH检测。利用这种独特的策略，探针在488 nm的单波长激发下显示出530和580 nm的双发射带。当pH值从3.0切换到8.0，酸解离常数（pKa）值为6.64时，探针的pH滴定率强度比（I530nm/I580nm）显著增加。该探针成功地应用于活细胞的成像和pH检测，而没有明显的细胞毒性，表明它是生物分析和生物成像的有前途的候选者。
Herein, we figured out a novel ratiometric fluorescent probe based on Janus silica nanoparticles (SiO2NPs) where fluorescein isothiocyanate (FITC) and rhodamine B (RB) were asymmetrically functionalized for intracellular pH detection. With this unique strategy, the probe exhibited dual emission bands at 530 and 580 nm upon excitation at a single wavelength of 488 nm. The pH titrations of the probe showed a remarkable increase in ratiometric intensities ratio (I530nm/I580nm) when the pH was switched from 3.0 to 8.0 with a acid dissociation constant (pKa) value of 6.64. The probe was successfully applied to imaging and detection of pH in living cells without noticeable cytotoxicity, indicating that it is a promising candidate for bioanalysis and bioimaging.