Identification of chromosome regions for high-temperature tolerance in the japonica rice cultivar ‘Genkitsukushi’ (Oryza sativa L.)
Identification of chromosome regions for high-temperature tolerance in the japonica rice cultivar ‘Genkitsukushi’ (Oryza sativa L.)
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DOI：
10.1080/1343943x.2022.2155669
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发表时间：
2022-12
影响因子：
2.5
通讯作者：
Katsunori Miyahara;Takuya Wada;S. Fukuoka;Masayuki Miyazaki;O. Yamaguchi;Masafumi Ishibashi;M. Kondo;N. Hamaoka;Yushi Ishibashi
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文献类型：
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作者：
Katsunori Miyahara;Takuya Wada;S. Fukuoka;Masayuki Miyazaki;O. Yamaguchi;Masafumi Ishibashi;M. Kondo;N. Hamaoka;Yushi Ishibashi
摘要由于成熟过程中的高温，水稻籽粒外观退化是一个严重的问题，全球变暖加剧了这一问题。为了有效培育新品种，必须对成熟过程中耐高温的QTL进行定位。在此，我们用耐高温的‘源氏’与高温敏感的‘筑人’杂交，育成了重组自交系。‘根结’的白背粒出现率显著低于‘筑本’。对它们的高温耐性进行了鉴定，并进行了数量性状基因座(QTL)分析。6号染色体上的白背粒QTL和大效应QTL。在为期两年的实验中鉴定出8种。CHR上的QTL。8与我们之前用来自‘Chikushi 52’בTsukushiroman’的rIL检测到的rIL接近。结果表明，CHR上的QTL。6为‘Genkitsukushi’בTsukushiroman’所特有，在CHR上也有。8可能是‘元气’和‘千斤52’之间的相同之处。在CHR上含有QTL区域的回交系。以《筑波人》为背景的《源氏物语》的8号验证了它的效果。我们还对CHR上QTL附近的标记基因型进行了分析。6个和8个在‘源氏’的亲属中。我们讨论了这些QTL之间的互作及其与高温耐性的关系。图形摘要
ABSTRACT Degradation of the appearance of rice grains due to high temperatures during ripening is a serious problem being worsened by global warming. QTLs for tolerance to high temperature during ripening must be identified in order to develop new cultivars efficiently. Here, we developed recombinant inbred lines (RILs) from crosses between the high-temperature-tolerant ‘Genkitsukushi’ and the high-temperature-sensitive ‘Tsukushiroman’. The occurrence of white-back grains is significantly lower in ‘Genkitsukushi’ than in ‘Tsukushiroman’. We evaluated their high-temperature tolerance and performed quantitative trait locus (QTL) analysis. A QTL for white-back grains on chromosome (chr.) 6 and a large-effect QTL on chr. 8 were identified in the two-year experiments. The QTL on chr. 8 was close to one we previously detected using RILs derived from ‘Chikushi 52’ × ‘Tsukushiroman’. The results indicate that the QTL on chr. 6 is unique to ‘Genkitsukushi’ × ‘Tsukushiroman’, and that on chr. 8 could be identical between ‘Genkitsukushi’ and ‘Chikushi 52’. Backcrossed lines which harbor the QTL region on chr. 8 of ‘Genkitsukushi’ in the background of ‘Tsukushiroman’ verified its effects. We also analyzed the marker genotypes near the QTLs on chr. 6 and 8 in relatives of ‘Genkitsukushi’. We discuss the interaction between these QTLs and their relationship with high-temperature tolerance. Graphical abstract