Mechanism underlying rapid responses to boron deprivation in Arabidopsis roots
Mechanism underlying rapid responses to boron deprivation in Arabidopsis roots
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DOI：
10.1080/00380768.2017.1416670
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发表时间：
2018-01-01
影响因子：
2
通讯作者：
Shibata, Daisuke
中科院分区：
文献类型：
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作者：
Kobayashi, Masaru;Miyamoto, Maako;Shibata, Daisuke
将水培生长的拟南芥（Arabidopsis thaliana L.）在不含硼（B）的培养基中培养1小时后，根尖细胞死亡。为了了解诱导的快速细胞死亡的机制，拟南芥响应B剥夺的特点是使用抑制剂，可能涉及的细胞过程。结果表明，质膜的拉伸和钙离子通过机械敏感性通道的内流触发的反应和活性氧在B剥夺条件下过量产生。此外，还观察到一氧化氮参与细胞死亡，这表明拟南芥根细胞在B剥夺后发生程序性细胞死亡。RNA测序分析显示，B剥夺诱导的转录组变化类似于病原体诱导的反应。综上所述，我们推测，B剥夺诱导拟南芥根过敏反应作为一种反应，有缺陷的细胞壁结构。
Transferring hydroponically grown Arabidopsis plants (Arabidopsis thaliana L.) to boron (B)-free medium kills the cells in root tips within an hour. To understand the mechanism underlying the induced rapid cell death, the Arabidopsis response to B deprivation was characterized using inhibitors of presumably involved cellular processes. The results suggest that stretching of the plasma membrane and the influx of calcium ions through mechanosensitive channels triggered the responses and that reactive oxygen species were overproduced under B-deprived condition. In addition, nitric oxide was observed to be involved in cell death, suggesting that Arabidopsis root cells undergo programmed cell death upon B deprivation. RNA sequencing analysis revealed that B deprivation induced transcriptome changes that resembled pathogen-induced responses. In summary, we speculate that B deprivation induces hypersensitive responses in Arabidopsis roots as a response to defective cell wall structures.