Phosphate and nitrate supplementations to evaluate the effect on cell biomass, intra and extracellular nodularin and nodulopeptin 901 produced by the cyanobacterium Nodularia spumigena KAC 66
Phosphate and nitrate supplementations to evaluate the effect on cell biomass, intra and extracellular nodularin and nodulopeptin 901 produced by the cyanobacterium Nodularia spumigena KAC 66
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DOI：
10.1007/s10811-019-02008-z
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发表时间：
2019-12-21
影响因子：
3.3
通讯作者：
Edwards, Christine
中科院分区：
文献类型：
--
作者：
Hameed, Shaista;Lawton, Linda A.;Edwards, Christine
海绵结节蓝藻的水华定期出现在波罗的海，通常产生广泛的生物活性多肽，包括肝毒素结节蛋白(NOD)、海绵蛋白、鱼腥藻多肽和结节多肽(分子量：917、901和899 Da)。本研究报道了从波罗的海分离的海绵微藻KAC 66在不同的硝酸盐和磷浓度下产生胞内和胞外NOD和结节肽901(主要次生代谢产物)的情况。以细胞生物量和细胞内外多肽为指标，用高效液相色谱-光电二极管阵列-质谱仪监测细胞生长情况。在本工作中，发现高浓度的硝酸盐和磷对海绵微藻的生物量和毒素水平有相当大的影响。与许多研究一样，在生长的5周内，细胞内保留了最大数量的结节。相反，在整个实验过程中，多达40%的结节肽901被排泄到培养液中。当L-1硝酸盐浓度为6.5和3.5 mg时，单位生物量多肽的最大浓度为1.78ngNod(第4周)和1.42ngNOD901mU g(-1)(第3周)。然而，在没有硝酸盐的情况下，这两种多肽的浓度都很高。磷酸盐实验表明菌体生长，多肽的产生依赖于磷的有效性。在磷酸盐浓度为502.4 mg/L时，胞内结节多肽901的含量(502.4 ng/g生物量)增加。这份报告评估了营养物质对生物量和毒素产生的影响，这可能会预测波罗的海海绵微藻水华的形成和控制。这也为在合适的条件下改善生长条件以产生高生物量和毒素提供了信息，这可能对研究有所帮助。目前的研究结果也将有助于根据硝酸盐和磷酸盐浓度的增加或减少来预测波罗的海可能发生的海绵微藻水华。
Blooms of the cyanobacterium Nodularia spumigena occur regularly in the Baltic Sea typically producing a wide range of bioactive peptides including the hepatotoxin nodularin (NOD), spumigins, anabaenopeptins and nodulopeptins (molecular weights: 917, 901 and 899 Da). This study reports the production of intracellular and extracellular NOD and nodulopeptin 901 (the major secondary metabolites), at various nitrate and phosphorus concentrations produced by N. spumigena KAC 66 which had been originally isolated from the Baltic Sea. The growth was observed by cell biomass and intracellular and extracellular peptides monitored by high-performance liquid chromatography with photodiode array and mass spectrometry (HPLC-PDA-MS). In the present work, it was found that high concentrations of nitrate and phosphorus have a considerable effect on biomass and toxin levels of N. spumigena. In common with many studies, the maximum amount of NOD was retained within the cells during 5 weeks of growth. In contrast, as much as similar to 40% of nodulopeptin 901 was excreted into the medium throughout the duration of experiments. At 6.5 and 3.5 mg L-1 nitrate, the maximum concentrations of peptide per unit biomass was 1.78 ng NOD (in week 4) and 1.42 ng nodulopeptin 901 mu g(-1) (in week 3) were detected. However, the high concentrations of both peptides were produced in the absence of nitrate. The phosphate experiment indicated growth, and peptide production was dependent on availability of phosphorus. At 0 mg L-1 of phosphate, an increased amount of intracellular (502.4 ng mu g(-1) biomass) nodulopeptin 901 was recorded. This report evaluates the effect of nutrients on the production of biomass and toxins, which may predict the formation and control of blooms of N. spumigena in the Baltic Sea. It also provides information to improve the growth conditions to produce high biomass and toxins under suitable conditions, which may be helpful in the research. The results from the current study will also be helpful to predict about possible blooms of N. spumigena in the Baltic Sea with reference to increase or decrease in nitrate and phosphate concentrations.