Expression analysis of genes encoding plasma membrane aquaporins during seed and fruit development in tomato
Expression analysis of genes encoding plasma membrane aquaporins during seed and fruit development in tomato
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DOI：
10.1016/j.plantsci.2006.03.021
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发表时间：
2006-08
期刊：
影响因子：
5.2
通讯作者：
H. Shiota;Takehiro Sudoh;I. Tanaka
中科院分区：
文献类型：
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作者：
H. Shiota;Takehiro Sudoh;I. Tanaka
水通道蛋白促进植物细胞生物膜的水通量，并参与多种植物组织中的各种生理现象。为了确定水通道蛋白在种子发育过程中是否具有生理作用，我们分析了番茄(Lycopersicesculentum Mill.)种子和果实发育过程中质膜内源蛋白(PIPs)编码基因的表达。根据保守的跨膜结构域和NPA基序，利用RT-PCR方法在成熟番茄种子中检测到6个编码PIPs的基因。用9个番茄PIPs的特异引物RT-PCR分析了这些基因在发育中的种子和果实中的表达，其中包括检测到的6个PIPs和来自番茄EST数据库的另外3个PIPs。在种子发育的早期[开花后12~32d]，7个PIP基因的表达增加，而在种子发育的后期(36~56d)，这些基因的表达下降。番茄PIP在果实发育过程中表现出不同的表达模式。此外，还计算了电池的含水率。种子水分含量在种子发育早期(12~32d)逐渐下降，36~56d维持在44~50%，而果实水分在整个果实发育过程中保持在90%左右。这些结果表明，质膜水孔蛋白在番茄种子和果实发育过程中起着重要的生理作用。
Aquaporins facilitate water flux across biomembranes in plant cells, and are involved in various physiological phenomena in several plant tissues. To determine whether aquaporins have physiological roles during seed development, we analyzed the expression of genes encoding plasma membrane intrinsic proteins (PIPs) during seed and fruit development in tomato (Lycopersicon esculentum Mill.). Six genes encoding PIPs were detected in mature tomato seeds by RT-PCR using PCR primers corresponding to conserved transmembrane domains and NPA motifs. The expression of these genes in developing seeds and fruit was analyzed by RT-PCR using primers specific for nine tomato PIPs, including the six PIPs detected and an additional three PIPs from the tomato EST database. Increased expression of seven PIPs was detected during the earlier phase of seed development [12–32 days after flowering (DAF)], and the expression of these genes decreased during the later phase (36–56 DAF). Each tomato PIP showed a distinct expression pattern during fruit development. In addition, the water content of the cells was calculated. The seed water content decreased gradually in the earlier phase of seed development (12–32 DAF), and was subsequently maintained at 44–50% from 36 to 56 DAF, whereas the water content of the fruit remained at 90% throughout fruit development. These results suggest that plasma membrane aquaporins play a physiological role during seed and fruit development in tomato.