Partition of lindane in synthetic and native membranes.
Partition of lindane in synthetic and native membranes.
复制标题
林丹在合成膜和天然膜中的分配。
DOI：
10.1016/0005-2736(85)90109-9
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
V. Madeira
中科院分区：
文献类型：
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作者：
M. C. Antunes;V. Madeira
测定了杀虫剂γ-1，2，3，4，5，6-六氯环己烷在模型膜和天然膜中的分配系数。在脂质处于液晶状态的范围（10-40°C）内，卵磷脂酰胆碱双层中的分配随温度线性降低。添加50 mol%胆固醇显著降低分配（2100福尔斯降至100，在10°C下）并消除温度依赖性。一级相变的二肉豆蔻酰，二棕榈酰和二硬脂酰磷脂酰胆碱（DMPC，DPPC和DSPC）的线性分配伴随着急剧增加。显然，杀虫剂很容易容纳在短脂族链脂质的双层中，因为在DMPC、DPPC和DSPC中，在低于其转变中点10 ℃的温度下，分区分别为2450、600和50。天然膜中的线性分配顺序如下：线粒体、肌浆网、髓磷脂、脑微粒体和红细胞。该序列与胆固醇的相对含量相当好地相关，并且在总提取脂质的脂质体中是相似的，尽管绝对分区显示出降低的值。因此，天然膜中蛋白质的存在有助于杀虫剂分配，可能是通过促进其与脂质的相互作用。
Partition coefficients of the insecticide γ-1,2,3,4,5,6-hexachlorocyclohexane (trivially, lindane) were determined in model and native membranes. Partition in egg phosphatidylcholine bilayers decreases linearly with temperature, over a range (10–40°C) at which the lipid is in the liquid-crystalline state. Addition of 50 mol% cholesterol dramatically decreases partition (2100 falls to 100, at 10°C) and abolishes the temperature dependence. First-order phase transitions of dimyristoyl-, dipalmitoyl- and distearoylphosphatidylcholines (DMPC, DPPC and DSPC) are accompanied by a sharp increase in lindane partition. Apparently, the insecticide is easily accommodated in bilayers of short-aliphatic-chain lipids, since the partitions were 2450, 600 and 50 in DMPC, DPPC and DSPC, respectively, at temperatures 10 Cdeg below the midpoint of their transitions. The lindane partition sequence in native membranes is as follows: mitochondria, sarcoplasmic reticulum, myelin, brain microsomes and erythrocytes. This sequence correlates reasonably well with the relative content of cholesterol and is similar in liposomes of total extracted lipids, although the absolute partitions showed decreased values. Therefore, the presence of proteins in native membranes contributes to the insecticide partition, probably by favouring its interaction with lipids.